Restriction map for
pFN21AE5286
(Linear) (Six-base) MAPSORT of: KIEE5286.seq Check: 4815 from: 1 to: 696
KIEE5286.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AfeI AGC'GCT
Cuts at: 0 164 696
Size: 164 532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 326 696
Size: 49 277 370
Fragments arranged by size:
370 277 49
AlwNI CAG_nnn'CTG
Cuts at: 0 181 534 696
Size: 181 353 162
Fragments arranged by size:
353 181 162
ApaI G_GGCC'C
Cuts at: 0 417 480 696
Size: 417 63 216
Fragments arranged by size:
417 216 63
ApaLI G'TGCA_C
Cuts at: 0 301 696
Size: 301 395
AvaI C'yCGr_G
Cuts at: 0 499 613 696
Size: 499 114 83
Fragments arranged by size:
499 114 83
BanI G'GyrC_C
Cuts at: 0 25 574 696
Size: 25 549 122
Fragments arranged by size:
549 122 25
BanII G_rGCy'C
Cuts at: 0 346 417 457 480 696
Size: 346 71 40 23 216
Fragments arranged by size:
346 216 71 40 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 633 662 696
Size: 633 29 34
Fragments arranged by size:
633 34 29
BlpI GC'TnA_GC
Cuts at: 0 340 696
Size: 340 356
Bme1580I G_kGCm'C
Cuts at: 0 305 417 480 577 696
Size: 305 112 63 97 119
Fragments arranged by size:
305 119 112 97 63
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 551 696
Size: 529 22 145
Fragments arranged by size:
529 145 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 696
Size: 360 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 490 610 654 696
Size: 247 243 120 44 42
Fragments arranged by size:
247 243 120 44 42
Bpu10I CC'TnA_GC
Cuts at: 0 169 696
Size: 169 527
BsaI GGTCTCn'nnnn_
Cuts at: 0 680 696
Size: 680 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 696
Size: 607 89
BseYI C'CCAG_C
Cuts at: 0 3 123 696
Size: 3 120 573
Fragments arranged by size:
573 120 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 366 696
Size: 233 133 330
Fragments arranged by size:
330 233 133
BsiEI CG_ry'CG
Cuts at: 0 81 696
Size: 81 615
BsiHKAI G_wGCw'C
Cuts at: 0 305 346 620 696
Size: 305 41 274 76
Fragments arranged by size:
305 274 76 41
Bsp1286I G_dGCh'C
Cuts at: 0 305 346 417 457 480 577 620 696
Size: 305 41 71 40 23 97 43 76
Fragments arranged by size:
305 97 76 71 43 41 40 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 668 696
Size: 146 522 28
Fragments arranged by size:
522 146 28
BsrBI CCG'CTC
Cuts at: 0 558 696
Size: 558 138
BsrFI r'CCGG_y
Cuts at: 0 22 696
Size: 22 674
BstYI r'GATC_y
Cuts at: 0 194 696
Size: 194 502
Bsu36I CC'TnA_GG
Cuts at: 0 118 696
Size: 118 578
BtgI C'CryG_G
Cuts at: 0 144 488 696
Size: 144 344 208
Fragments arranged by size:
344 208 144
EaeI y'GGCC_r
Cuts at: 0 63 428 696
Size: 63 365 268
Fragments arranged by size:
365 268 63
EcoICRI GAG'CTC
Cuts at: 0 344 696
Size: 344 352
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 490 610 654 696
Size: 247 243 120 44 42
Fragments arranged by size:
247 243 120 44 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 696
Size: 592 104
EcoO109I rG'GnC_Cy
Cuts at: 0 242 322 477 696
Size: 242 80 155 219
Fragments arranged by size:
242 219 155 80
HaeII r_GCGC'y
Cuts at: 0 166 696
Size: 166 530
HindIII A'AGCT_T
Cuts at: 0 582 696
Size: 582 114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 696
Size: 651 45
MscI TGG'CCA
Cuts at: 0 430 696
Size: 430 266
MslI CAynn'nnrTG
Cuts at: 0 31 275 332 696
Size: 31 244 57 364
Fragments arranged by size:
364 244 57 31
MspA1I CmG'CkG
Cuts at: 0 259 534 696
Size: 259 275 162
Fragments arranged by size:
275 259 162
NaeI GCC'GGC
Cuts at: 0 24 696
Size: 24 672
NgoMIV G'CCGG_C
Cuts at: 0 22 696
Size: 22 674
PfoI T'CCnGG_A
Cuts at: 0 206 587 696
Size: 206 381 109
Fragments arranged by size:
381 206 109
PpuMI rG'GwC_Cy
Cuts at: 0 242 322 696
Size: 242 80 374
Fragments arranged by size:
374 242 80
PspOMI G'GGCC_C
Cuts at: 0 413 476 696
Size: 413 63 220
Fragments arranged by size:
413 220 63
PstI C_TGCA'G
Cuts at: 0 530 696
Size: 530 166
PvuII CAG'CTG
Cuts at: 0 259 696
Size: 259 437
SacI G_AGCT'C
Cuts at: 0 346 696
Size: 346 350
SanDI GG'GwC_CC
Cuts at: 0 242 322 696
Size: 242 80 374
Fragments arranged by size:
374 242 80
SfcI C'TryA_G
Cuts at: 0 526 696
Size: 526 170
SmlI C'TyrA_G
Cuts at: 0 613 696
Size: 613 83
StuI AGG'CCT
Cuts at: 0 94 232 696
Size: 94 138 464
Fragments arranged by size:
464 138 94
StyI C'CwwG_G
Cuts at: 0 54 696
Size: 54 642
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 267 696
Size: 267 429
Tth111I GACn'n_nGTC
Cuts at: 0 637 696
Size: 637 59
XhoI C'TCGA_G
Cuts at: 0 613 696
Size: 613 83
Enzymes that do cut:
AfeI AlfI AlwNI ApaI ApaLI AvaI BanI BanII
BbsI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstYI Bsu36I BtgI EaeI EcoICRI Eco57MI EcoNI
EcoO109I HaeII HindIII MmeI MscI MslI MspA1I NaeI
NgoMIV PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SfcI SmlI StuI StyI TaqII Tth111I XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AloI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
XbaI XcmI XmaI XmnI ZraI