Restriction map for
pFN21AE5284
(Linear) (Six-base) MAPSORT of: KIEE5284.seq Check: 9362 from: 1 to: 630
KIEE5284.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 513 630
Size: 513 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 630
Size: 166 464
AleI CACnn'nnGTG
Cuts at: 0 305 630
Size: 305 325
AlwNI CAG_nnn'CTG
Cuts at: 0 246 471 630
Size: 246 225 159
Fragments arranged by size:
246 225 159
ApaI G_GGCC'C
Cuts at: 0 483 513 630
Size: 483 30 117
Fragments arranged by size:
483 117 30
AvaI C'yCGr_G
Cuts at: 0 129 482 630
Size: 129 353 148
Fragments arranged by size:
353 148 129
BanII G_rGCy'C
Cuts at: 0 483 513 630
Size: 483 30 117
Fragments arranged by size:
483 117 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 630
Size: 154 476
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 120 154 564 598 630
Size: 120 34 410 34 32
Fragments arranged by size:
410 120 34 34 32
BglII A'GATC_T
Cuts at: 0 119 378 392 630
Size: 119 259 14 238
Fragments arranged by size:
259 238 119 14
Bme1580I G_kGCm'C
Cuts at: 0 483 513 630
Size: 483 30 117
Fragments arranged by size:
483 117 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 268 630
Size: 202 66 362
Fragments arranged by size:
362 202 66
Bpu10I CC'TnA_GC
Cuts at: 0 488 630
Size: 488 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 630
Size: 502 128
BsaHI Gr'CG_yC
Cuts at: 0 60 630
Size: 60 570
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 211 630
Size: 57 154 419
Fragments arranged by size:
419 154 57
BseYI C'CCAG_C
Cuts at: 0 261 474 630
Size: 261 213 156
Fragments arranged by size:
261 213 156
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 26 630
Size: 26 604
BsiHKAI G_wGCw'C
Cuts at: 0 232 630
Size: 232 398
Bsp1286I G_dGCh'C
Cuts at: 0 232 483 513 630
Size: 232 251 30 117
Fragments arranged by size:
251 232 117 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 512 513 630
Size: 512 1 117
Fragments arranged by size:
512 117 1
BsrBI CCG'CTC
Cuts at: 0 65 201 630
Size: 65 136 429
Fragments arranged by size:
429 136 65
BssHII G'CGCG_C
Cuts at: 0 219 630
Size: 219 411
BstAPI GCAn_nnn'nTGC
Cuts at: 0 236 471 630
Size: 236 235 159
Fragments arranged by size:
236 235 159
BstYI r'GATC_y
Cuts at: 0 119 378 392 443 630
Size: 119 259 14 51 187
Fragments arranged by size:
259 187 119 51 14
BtgI C'CryG_G
Cuts at: 0 89 630
Size: 89 541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 32 630
Size: 32 598
ClaI AT'CG_AT
Cuts at: 0 584 630
Size: 584 46
DraIII CAC_nnn'GTG
Cuts at: 0 595 630
Size: 595 35
EaeI y'GGCC_r
Cuts at: 0 254 630
Size: 254 376
EarI CTCTTCn'nnn_
Cuts at: 0 320 630
Size: 320 310
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 114 208 630
Size: 19 95 94 422
Fragments arranged by size:
422 95 94 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 202 268 630
Size: 166 36 66 362
Fragments arranged by size:
362 166 66 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 520 630
Size: 520 110
EcoO109I rG'GnC_Cy
Cuts at: 0 479 630
Size: 479 151
HaeII r_GCGC'y
Cuts at: 0 472 630
Size: 472 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 363 395 428 460 630
Size: 363 32 33 32 170
Fragments arranged by size:
363 170 33 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 630
Size: 95 535
MslI CAynn'nnrTG
Cuts at: 0 305 630
Size: 305 325
MspA1I CmG'CkG
Cuts at: 0 144 236 246 630
Size: 144 92 10 384
Fragments arranged by size:
384 144 92 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 630
Size: 246 384
PspOMI G'GGCC_C
Cuts at: 0 479 509 630
Size: 479 30 121
Fragments arranged by size:
479 121 30
PstI C_TGCA'G
Cuts at: 0 164 281 453 630
Size: 164 117 172 177
Fragments arranged by size:
177 172 164 117
PvuII CAG'CTG
Cuts at: 0 144 630
Size: 144 486
SfcI C'TryA_G
Cuts at: 0 160 277 449 630
Size: 160 117 172 181
Fragments arranged by size:
181 172 160 117
SmaI CCC'GGG
Cuts at: 0 484 630
Size: 484 146
SmlI C'TyrA_G
Cuts at: 0 129 517 630
Size: 129 388 113
Fragments arranged by size:
388 129 113
SrfI GCCC'GGGC
Cuts at: 0 484 630
Size: 484 146
XhoI C'TCGA_G
Cuts at: 0 129 630
Size: 129 501
XmaI C'CCGG_G
Cuts at: 0 482 630
Size: 482 148
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BanII BbsI
BcgI BglII Bme1580I BpmI Bpu10I BpuEI BsaHI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BssHII BstAPI
BstYI BtgI BtgZI ClaI DraIII EaeI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I MmeI MslI MspA1I
PflMI PspOMI PstI PvuII SfcI SmaI SmlI SrfI
XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI CspCI DraI DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XmnI ZraI