Restriction map for
pFN21AE5280
(Linear) (Six-base) MAPSORT of: KIEE5280.seq Check: 1390 from: 1 to: 744
KIEE5280.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 421 744
Size: 421 323
Acc65I G'GTAC_C
Cuts at: 0 242 744
Size: 242 502
AflIII A'CryG_T
Cuts at: 0 101 744
Size: 101 643
AleI CACnn'nnGTG
Cuts at: 0 422 744
Size: 422 322
ApaI G_GGCC'C
Cuts at: 0 590 744
Size: 590 154
AvaI C'yCGr_G
Cuts at: 0 323 744
Size: 323 421
BanI G'GyrC_C
Cuts at: 0 242 744
Size: 242 502
BanII G_rGCy'C
Cuts at: 0 321 590 744
Size: 321 269 154
Fragments arranged by size:
321 269 154
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 744
Size: 209 535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 456 490 744
Size: 456 34 254
Fragments arranged by size:
456 254 34
BglI GCCn_nnn'nGGC
Cuts at: 0 293 744
Size: 293 451
BglII A'GATC_T
Cuts at: 0 541 610 727 744
Size: 541 69 117 17
Fragments arranged by size:
541 117 69 17
Bme1580I G_kGCm'C
Cuts at: 0 590 744
Size: 590 154
BmgBI CAC'GTC
Cuts at: 0 102 744
Size: 102 642
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 744
Size: 56 688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 744
Size: 124 620
BsaAI yAC'GTr
Cuts at: 0 57 744
Size: 57 687
BsaBI GATnn'nnATC
Cuts at: 0 615 744
Size: 615 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 29 657 687 744
Size: 29 628 30 57
Fragments arranged by size:
628 57 30 29
BsiEI CG_ry'CG
Cuts at: 0 276 744
Size: 276 468
BsiHKAI G_wGCw'C
Cuts at: 0 321 744
Size: 321 423
BsmBI CGTCTCn'nnnn_
Cuts at: 0 188 744
Size: 188 556
Bsp1286I G_dGCh'C
Cuts at: 0 321 590 744
Size: 321 269 154
Fragments arranged by size:
321 269 154
BspHI T'CATG_A
Cuts at: 0 546 744
Size: 546 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 421 744
Size: 421 323
BstBI TT'CG_AA
Cuts at: 0 516 744
Size: 516 228
BstYI r'GATC_y
Cuts at: 0 541 610 727 744
Size: 541 69 117 17
Fragments arranged by size:
541 117 69 17
BtgI C'CryG_G
Cuts at: 0 150 276 744
Size: 150 126 468
Fragments arranged by size:
468 150 126
DraI TTT'AAA
Cuts at: 0 410 744
Size: 410 334
EaeI y'GGCC_r
Cuts at: 0 273 744
Size: 273 471
EagI C'GGCC_G
Cuts at: 0 273 744
Size: 273 471
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 329 744
Size: 329 415
EcoICRI GAG'CTC
Cuts at: 0 319 744
Size: 319 425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 744
Size: 56 688
EcoO109I rG'GnC_Cy
Cuts at: 0 186 744
Size: 186 558
HaeII r_GCGC'y
Cuts at: 0 122 744
Size: 122 622
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 356 388 744
Size: 356 32 356
Fragments arranged by size:
356 356 32
KpnI G_GTAC'C
Cuts at: 0 246 744
Size: 246 498
MslI CAynn'nnrTG
Cuts at: 0 55 422 744
Size: 55 367 322
Fragments arranged by size:
367 322 55
MspA1I CmG'CkG
Cuts at: 0 152 744
Size: 152 592
NruI TCG'CGA
Cuts at: 0 221 744
Size: 221 523
NspI r_CATG'y
Cuts at: 0 423 744
Size: 423 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 604 744
Size: 604 140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 744
Size: 88 32 624
Fragments arranged by size:
624 88 32
PpuMI rG'GwC_Cy
Cuts at: 0 186 744
Size: 186 558
PspOMI G'GGCC_C
Cuts at: 0 586 744
Size: 586 158
SacI G_AGCT'C
Cuts at: 0 321 744
Size: 321 423
SacII CC_GC'GG
Cuts at: 0 153 744
Size: 153 591
SmlI C'TyrA_G
Cuts at: 0 139 744
Size: 139 605
StyI C'CwwG_G
Cuts at: 0 86 682 744
Size: 86 596 62
Fragments arranged by size:
596 86 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 744
Size: 31 713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 395 744
Size: 395 349
Enzymes that do cut:
AarI Acc65I AflIII AleI ApaI AvaI BanI BanII
BbsI BcgI BglI BglII Bme1580I BmgBI BpmI BpuEI
BsaAI BsaBI BsaXI BsiEI BsiHKAI BsmBI Bsp1286I BspHI
BspMI BstBI BstYI BtgI DraI EaeI EagI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MslI MspA1I
NruI NspI PflMI PpiI PpuMI PspOMI SacI SacII
SmlI StyI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI
BlpI BmrI BmtI BplI Bpu10I BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiWI BsmI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EarI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI