Restriction map for
pFN21AE5277
(Linear) (Six-base) MAPSORT of: KIEE5277.seq Check: 1060 from: 1 to: 660
KIEE5277.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 660
Size: 400 260
AvaI C'yCGr_G
Cuts at: 0 108 660
Size: 108 552
BanI G'GyrC_C
Cuts at: 0 407 660
Size: 407 253
BanII G_rGCy'C
Cuts at: 0 443 660
Size: 443 217
BbsI GAAGACnn'nnnn_
Cuts at: 0 228 246 660
Size: 228 18 414
Fragments arranged by size:
414 228 18
BbvCI CC'TCA_GC
Cuts at: 0 334 660
Size: 334 326
BglI GCCn_nnn'nGGC
Cuts at: 0 422 660
Size: 422 238
Bme1580I G_kGCm'C
Cuts at: 0 212 660
Size: 212 448
BmgBI CAC'GTC
Cuts at: 0 558 660
Size: 558 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 372 660
Size: 372 288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 491 660
Size: 491 169
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 660
Size: 552 108
Bpu10I CC'TnA_GC
Cuts at: 0 334 660
Size: 334 326
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 165 660
Size: 85 80 495
Fragments arranged by size:
495 85 80
BseYI C'CCAG_C
Cuts at: 0 162 652 660
Size: 162 490 8
Fragments arranged by size:
490 162 8
BsiHKAI G_wGCw'C
Cuts at: 0 329 660
Size: 329 331
BsmBI CGTCTCn'nnnn_
Cuts at: 0 85 362 660
Size: 85 277 298
Fragments arranged by size:
298 277 85
Bsp1286I G_dGCh'C
Cuts at: 0 212 329 443 660
Size: 212 117 114 217
Fragments arranged by size:
217 212 117 114
BsrBI CCG'CTC
Cuts at: 0 223 660
Size: 223 437
BsrFI r'CCGG_y
Cuts at: 0 404 660
Size: 404 256
BstBI TT'CG_AA
Cuts at: 0 144 660
Size: 144 516
BstYI r'GATC_y
Cuts at: 0 398 660
Size: 398 262
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 177 660
Size: 177 483
ClaI AT'CG_AT
Cuts at: 0 17 660
Size: 17 643
DraIII CAC_nnn'GTG
Cuts at: 0 347 660
Size: 347 313
DrdI GACnn_nn'nnGTC
Cuts at: 0 232 660
Size: 232 428
EaeI y'GGCC_r
Cuts at: 0 584 660
Size: 584 76
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 552 660
Size: 400 152 108
Fragments arranged by size:
400 152 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 295 660
Size: 67 228 365
Fragments arranged by size:
365 228 67
EcoO109I rG'GnC_Cy
Cuts at: 0 105 187 375 660
Size: 105 82 188 285
Fragments arranged by size:
285 188 105 82
EcoRV GAT'ATC
Cuts at: 0 15 660
Size: 15 645
MfeI C'AATT_G
Cuts at: 0 514 660
Size: 514 146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 660
Size: 78 582
MscI TGG'CCA
Cuts at: 0 586 660
Size: 586 74
NspI r_CATG'y
Cuts at: 0 390 660
Size: 390 270
PasI CC'CwG_GG
Cuts at: 0 418 660
Size: 418 242
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 660
Size: 96 564
PpuMI rG'GwC_Cy
Cuts at: 0 105 187 660
Size: 105 82 473
Fragments arranged by size:
473 105 82
SanDI GG'GwC_CC
Cuts at: 0 105 187 660
Size: 105 82 473
Fragments arranged by size:
473 105 82
SexAI A'CCwGG_T
Cuts at: 0 290 660
Size: 290 370
SmaI CCC'GGG
Cuts at: 0 110 660
Size: 110 550
StyI C'CwwG_G
Cuts at: 0 212 660
Size: 212 448
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 660
Size: 339 321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 49 81 660
Size: 49 32 579
Fragments arranged by size:
579 49 32
XmaI C'CCGG_G
Cuts at: 0 108 660
Size: 108 552
Enzymes that do cut:
AcuI AvaI BanI BanII BbsI BbvCI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BseRI BseYI BsiHKAI
BsmBI Bsp1286I BsrBI BsrFI BstBI BstYI BtgZI ClaI
DraIII DrdI EaeI Eco57MI EcoNI EcoO109I EcoRV MfeI
MmeI MscI NspI PasI PflMI PpuMI SanDI SexAI
SmaI StyI TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglII BlpI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BspEI BspHI BspMI BsrDI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgI BtsI
CspCI DraI EagI EarI EciI EcoICRI EcoRI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI XmnI ZraI