Restriction map for
pFN21AE5271
(Linear) (Six-base) MAPSORT of: KIEE5271.seq Check: 9694 from: 1 to: 654
KIEE5271.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 232 654
Size: 172 60 422
Fragments arranged by size:
422 172 60
AfeI AGC'GCT
Cuts at: 0 91 654
Size: 91 563
AhdI GACnn_n'nnGTC
Cuts at: 0 627 654
Size: 627 27
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 654
Size: 278 32 344
Fragments arranged by size:
344 278 32
AlwNI CAG_nnn'CTG
Cuts at: 0 207 654
Size: 207 447
AvaI C'yCGr_G
Cuts at: 0 313 484 654
Size: 313 171 170
Fragments arranged by size:
313 171 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 410 654
Size: 410 244
BglI GCCn_nnn'nGGC
Cuts at: 0 142 654
Size: 142 512
BglII A'GATC_T
Cuts at: 0 557 654
Size: 557 97
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 346 555 654
Size: 106 240 209 99
Fragments arranged by size:
240 209 106 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 654
Size: 28 626
BsaI GGTCTCn'nnnn_
Cuts at: 0 322 654
Size: 322 332
BsaBI GATnn'nnATC
Cuts at: 0 562 654
Size: 562 92
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 636 654
Size: 636 18
BseYI C'CCAG_C
Cuts at: 0 171 654
Size: 171 483
BsmBI CGTCTCn'nnnn_
Cuts at: 0 94 654
Size: 94 560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 340 654
Size: 329 11 314
Fragments arranged by size:
329 314 11
BstEII G'GTnAC_C
Cuts at: 0 192 654
Size: 192 462
BstXI CCAn_nnnn'nTGG
Cuts at: 0 639 654
Size: 639 15
BstYI r'GATC_y
Cuts at: 0 203 489 557 654
Size: 203 286 68 97
Fragments arranged by size:
286 203 97 68
BtgI C'CryG_G
Cuts at: 0 188 654
Size: 188 466
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 19 654
Size: 19 635
DraIII CAC_nnn'GTG
Cuts at: 0 251 654
Size: 251 403
EaeI y'GGCC_r
Cuts at: 0 77 289 473 654
Size: 77 212 184 181
Fragments arranged by size:
212 184 181 77
EarI CTCTTCn'nnn_
Cuts at: 0 572 654
Size: 572 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 172 232 346 555 654
Size: 106 66 60 114 209 99
Fragments arranged by size:
209 114 106 99 66 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 211 654
Size: 211 443
EcoO109I rG'GnC_Cy
Cuts at: 0 216 318 654
Size: 216 102 336
Fragments arranged by size:
336 216 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 633 654
Size: 633 21
HaeII r_GCGC'y
Cuts at: 0 93 654
Size: 93 561
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 654
Size: 613 32 9
Fragments arranged by size:
613 32 9
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 321 476 654
Size: 321 155 178
Fragments arranged by size:
321 178 155
MscI TGG'CCA
Cuts at: 0 79 654
Size: 79 575
MspA1I CmG'CkG
Cuts at: 0 464 654
Size: 464 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 654
Size: 435 219
PpuMI rG'GwC_Cy
Cuts at: 0 318 654
Size: 318 336
PshAI GACnn'nnGTC
Cuts at: 0 190 654
Size: 190 464
SfcI C'TryA_G
Cuts at: 0 567 654
Size: 567 87
SmlI C'TyrA_G
Cuts at: 0 43 654
Size: 43 611
StuI AGG'CCT
Cuts at: 0 346 654
Size: 346 308
StyI C'CwwG_G
Cuts at: 0 110 654
Size: 110 544
Tth111I GACn'n_nGTC
Cuts at: 0 409 654
Size: 409 245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 654
Size: 600 54
Enzymes that do cut:
AcuI AfeI AhdI AloI AlwNI AvaI BbsI BglI
BglII BpmI BpuEI BsaI BsaBI BseRI BseYI BsmBI
BspMI BstEII BstXI BstYI BtgI BtgZI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI
MscI MspA1I PflMI PpuMI PshAI SfcI SmlI StuI
StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaHI BsaWI BsaXI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI TstI XbaI XhoI XmaI XmnI
ZraI