Restriction map for
pFN21AE5270
(Linear) (Six-base) MAPSORT of: KIEE5270.seq Check: 1594 from: 1 to: 624
KIEE5270.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
Acc65I G'GTAC_C
Cuts at: 0 554 624
Size: 554 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 624
Size: 46 578
AvaI C'yCGr_G
Cuts at: 0 405 624
Size: 405 219
AvrII C'CTAG_G
Cuts at: 0 156 624
Size: 156 468
BanI G'GyrC_C
Cuts at: 0 554 624
Size: 554 70
BanII G_rGCy'C
Cuts at: 0 380 387 624
Size: 380 7 237
Fragments arranged by size:
380 237 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 316 624
Size: 316 308
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 66 624
Size: 32 34 558
Fragments arranged by size:
558 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 144 624
Size: 144 480
BglI GCCn_nnn'nGGC
Cuts at: 0 496 624
Size: 496 128
BglII A'GATC_T
Cuts at: 0 201 624
Size: 201 423
BlpI GC'TnA_GC
Cuts at: 0 436 624
Size: 436 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 439 624
Size: 439 185
BmtI G_CTAG'C
Cuts at: 0 100 624
Size: 100 524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 624
Size: 97 527
BsaWI w'CCGG_w
Cuts at: 0 173 624
Size: 173 451
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 555 585 624
Size: 555 30 39
Fragments arranged by size:
555 39 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 581 624
Size: 280 301 43
Fragments arranged by size:
301 280 43
BsiEI CG_ry'CG
Cuts at: 0 168 624
Size: 168 456
BsiHKAI G_wGCw'C
Cuts at: 0 272 380 624
Size: 272 108 244
Fragments arranged by size:
272 244 108
BsmI GAATG_Cn'
Cuts at: 0 364 624
Size: 364 260
Bsp1286I G_dGCh'C
Cuts at: 0 272 380 387 624
Size: 272 108 7 237
Fragments arranged by size:
272 237 108 7
BsrBI CCG'CTC
Cuts at: 0 81 624
Size: 81 543
BstEII G'GTnAC_C
Cuts at: 0 409 624
Size: 409 215
BstYI r'GATC_y
Cuts at: 0 201 624
Size: 201 423
BtgI C'CryG_G
Cuts at: 0 137 351 414 491 624
Size: 137 214 63 77 133
Fragments arranged by size:
214 137 133 77 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 178 624
Size: 178 446
EaeI y'GGCC_r
Cuts at: 0 165 248 488 624
Size: 165 83 240 136
Fragments arranged by size:
240 165 136 83
EagI C'GGCC_G
Cuts at: 0 165 624
Size: 165 459
EarI CTCTTCn'nnn_
Cuts at: 0 304 584 624
Size: 304 280 40
Fragments arranged by size:
304 280 40
EcoICRI GAG'CTC
Cuts at: 0 378 624
Size: 378 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 97 624
Size: 46 51 527
Fragments arranged by size:
527 51 46
EcoO109I rG'GnC_Cy
Cuts at: 0 315 624
Size: 315 309
KpnI G_GTAC'C
Cuts at: 0 558 624
Size: 558 66
MscI TGG'CCA
Cuts at: 0 250 624
Size: 250 374
MspA1I CmG'CkG
Cuts at: 0 147 624
Size: 147 477
NcoI C'CATG_G
Cuts at: 0 351 414 624
Size: 351 63 210
Fragments arranged by size:
351 210 63
NheI G'CTAG_C
Cuts at: 0 96 624
Size: 96 528
PpuMI rG'GwC_Cy
Cuts at: 0 315 624
Size: 315 309
PshAI GACnn'nnGTC
Cuts at: 0 320 624
Size: 320 304
PstI C_TGCA'G
Cuts at: 0 45 624
Size: 45 579
PvuII CAG'CTG
Cuts at: 0 147 624
Size: 147 477
SacI G_AGCT'C
Cuts at: 0 380 624
Size: 380 244
SanDI GG'GwC_CC
Cuts at: 0 315 624
Size: 315 309
SfcI C'TryA_G
Cuts at: 0 41 624
Size: 41 583
SmaI CCC'GGG
Cuts at: 0 407 624
Size: 407 217
StyI C'CwwG_G
Cuts at: 0 156 351 414 624
Size: 156 195 63 210
Fragments arranged by size:
210 195 156 63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 168 200 624
Size: 168 32 424
Fragments arranged by size:
424 168 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 624
Size: 489 135
XmaI C'CCGG_G
Cuts at: 0 405 624
Size: 405 219
Enzymes that do cut:
Acc65I AcuI AvaI AvrII BanI BanII BbsI BcgI
BciVI BglI BglII BlpI BmrI BmtI BpmI BsaWI
BsaXI BseRI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BstEII
BstYI BtgI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoO109I KpnI MscI MspA1I NcoI NheI PpuMI PshAI
PstI PvuII SacI SanDI SfcI SmaI StyI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BbeI BbvCI BclI
BfrBI Bme1580I BmgBI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BseYI BsgI BsiWI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI MmeI MslI NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI
XmnI ZraI