Restriction map for
pFN21AE5268
(Linear) (Six-base) MAPSORT of: KIEE5268.seq Check: 8220 from: 1 to: 603
KIEE5268.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AccI GT'mk_AC
Cuts at: 0 89 603
Size: 89 514
AfeI AGC'GCT
Cuts at: 0 286 340 603
Size: 286 54 263
Fragments arranged by size:
286 263 54
ApaI G_GGCC'C
Cuts at: 0 197 208 271 603
Size: 197 11 63 332
Fragments arranged by size:
332 197 63 11
ApoI r'AATT_y
Cuts at: 0 596 603
Size: 596 7
AvaI C'yCGr_G
Cuts at: 0 208 272 603
Size: 208 64 331
Fragments arranged by size:
331 208 64
BanI G'GyrC_C
Cuts at: 0 402 603
Size: 402 201
BanII G_rGCy'C
Cuts at: 0 70 197 208 271 499 603
Size: 70 127 11 63 228 104
Fragments arranged by size:
228 127 104 70 63 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 580 603
Size: 580 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 233 603
Size: 199 34 370
Fragments arranged by size:
370 199 34
Bme1580I G_kGCm'C
Cuts at: 0 197 208 271 407 603
Size: 197 11 63 136 196
Fragments arranged by size:
197 196 136 63 11
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 84 395 398 424 521 603
Size: 54 30 311 3 26 97 82
Fragments arranged by size:
311 97 82 54 30 26 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 603
Size: 453 150
BsaAI yAC'GTr
Cuts at: 0 84 603
Size: 84 519
BsaHI Gr'CG_yC
Cuts at: 0 128 603
Size: 128 475
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 116 146 454 484 603
Size: 82 30 4 30 308 30 119
Fragments arranged by size:
308 119 82 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 603
Size: 137 466
BseYI C'CCAG_C
Cuts at: 0 141 287 541 603
Size: 141 146 254 62
Fragments arranged by size:
254 146 141 62
BsiHKAI G_wGCw'C
Cuts at: 0 298 603
Size: 298 305
BsmBI CGTCTCn'nnnn_
Cuts at: 0 119 205 603
Size: 119 86 398
Fragments arranged by size:
398 119 86
Bsp1286I G_dGCh'C
Cuts at: 0 70 197 208 271 298 407 499 603
Size: 70 127 11 63 27 109 92 104
Fragments arranged by size:
127 109 104 92 70 63 27 11
BsrGI T'GTAC_A
Cuts at: 0 224 603
Size: 224 379
BstXI CCAn_nnnn'nTGG
Cuts at: 0 490 603
Size: 490 113
Bsu36I CC'TnA_GG
Cuts at: 0 200 603
Size: 200 403
BtgI C'CryG_G
Cuts at: 0 131 603
Size: 131 472
EaeI y'GGCC_r
Cuts at: 0 238 309 423 603
Size: 238 71 114 180
Fragments arranged by size:
238 180 114 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 603
Size: 67 536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 84 395 398 424 521 603
Size: 54 30 311 3 26 97 82
Fragments arranged by size:
311 97 82 54 30 26 3
EcoO109I rG'GnC_Cy
Cuts at: 0 193 194 204 205 267 268 603
Size: 193 1 10 1 62 1 335
Fragments arranged by size:
335 193 62 10 1 1 1
EcoRI G'AATT_C
Cuts at: 0 596 603
Size: 596 7
HaeII r_GCGC'y
Cuts at: 0 288 342 603
Size: 288 54 261
Fragments arranged by size:
288 261 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 454 486 570 602 603
Size: 454 32 84 32 1
Fragments arranged by size:
454 84 32 32 1
MscI TGG'CCA
Cuts at: 0 240 311 425 603
Size: 240 71 114 178
Fragments arranged by size:
240 178 114 71
MslI CAynn'nnrTG
Cuts at: 0 400 519 603
Size: 400 119 84
Fragments arranged by size:
400 119 84
PasI CC'CwG_GG
Cuts at: 0 190 603
Size: 190 413
PspOMI G'GGCC_C
Cuts at: 0 193 204 267 603
Size: 193 11 63 336
Fragments arranged by size:
336 193 63 11
SmaI CCC'GGG
Cuts at: 0 274 603
Size: 274 329
SmlI C'TyrA_G
Cuts at: 0 432 603
Size: 432 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 279 603
Size: 279 324
TatI w'GTAC_w
Cuts at: 0 224 603
Size: 224 379
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 603
Size: 116 32 455
Fragments arranged by size:
455 116 32
Tth111I GACn'n_nGTC
Cuts at: 0 166 603
Size: 166 437
XmaI C'CCGG_G
Cuts at: 0 272 603
Size: 272 331
Enzymes that do cut:
AccI AfeI ApaI ApoI AvaI BanI BanII BbsI
BcgI Bme1580I BpmI BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BsrGI BstXI Bsu36I BtgI
EaeI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I MscI
MslI PasI PspOMI SmaI SmlI TaqII TatI TstI
Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI XbaI XcmI XhoI
XmnI ZraI