Restriction map for
pFN21AE5258
(Linear) (Six-base) MAPSORT of: KIEE5258.seq Check: 2305 from: 1 to: 768
KIEE5258.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AatII G_ACGT'C
Cuts at: 0 683 768
Size: 683 85
AflIII A'CryG_T
Cuts at: 0 385 768
Size: 385 383
AhdI GACnn_n'nnGTC
Cuts at: 0 637 768
Size: 637 131
AleI CACnn'nnGTG
Cuts at: 0 574 768
Size: 574 194
ApaLI G'TGCA_C
Cuts at: 0 83 768
Size: 83 685
AvaI C'yCGr_G
Cuts at: 0 269 399 768
Size: 269 130 369
Fragments arranged by size:
369 269 130
BanI G'GyrC_C
Cuts at: 0 557 768
Size: 557 211
BanII G_rGCy'C
Cuts at: 0 469 768
Size: 469 299
BbeI G_GCGC'C
Cuts at: 0 561 768
Size: 561 207
Bme1580I G_kGCm'C
Cuts at: 0 87 768
Size: 87 681
BmgBI CAC'GTC
Cuts at: 0 266 768
Size: 266 502
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 768
Size: 174 594
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 639 768
Size: 639 129
BsaHI Gr'CG_yC
Cuts at: 0 558 680 768
Size: 558 122 88
Fragments arranged by size:
558 122 88
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1 768
Size: 1 767
BseYI C'CCAG_C
Cuts at: 0 95 425 460 768
Size: 95 330 35 308
Fragments arranged by size:
330 308 95 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 768
Size: 602 166
BsiHKAI G_wGCw'C
Cuts at: 0 87 224 768
Size: 87 137 544
Fragments arranged by size:
544 137 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 272 768
Size: 272 496
Bsp1286I G_dGCh'C
Cuts at: 0 87 224 469 768
Size: 87 137 245 299
Fragments arranged by size:
299 245 137 87
BspHI T'CATG_A
Cuts at: 0 200 768
Size: 200 568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 768
Size: 236 532
BssHII G'CGCG_C
Cuts at: 0 298 768
Size: 298 470
BstAPI GCAn_nnn'nTGC
Cuts at: 0 228 768
Size: 228 540
BtgI C'CryG_G
Cuts at: 0 100 371 572 768
Size: 100 271 201 196
Fragments arranged by size:
271 201 196 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 254 768
Size: 254 514
BtsI GCAGTG_nn'
Cuts at: 0 183 768
Size: 183 585
DraIII CAC_nnn'GTG
Cuts at: 0 168 768
Size: 168 600
EarI CTCTTCn'nnn_
Cuts at: 0 754 768
Size: 754 14
EcoO109I rG'GnC_Cy
Cuts at: 0 429 631 768
Size: 429 202 137
Fragments arranged by size:
429 202 137
FspI TGC'GCA
Cuts at: 0 232 768
Size: 232 536
HaeII r_GCGC'y
Cuts at: 0 146 561 768
Size: 146 415 207
Fragments arranged by size:
415 207 146
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 252 284 623 655 768
Size: 252 32 339 32 113
Fragments arranged by size:
339 252 113 32 32
KasI G'GCGC_C
Cuts at: 0 557 768
Size: 557 211
MluI A'CGCG_T
Cuts at: 0 385 768
Size: 385 383
MslI CAynn'nnrTG
Cuts at: 0 205 574 768
Size: 205 369 194
Fragments arranged by size:
369 205 194
MspA1I CmG'CkG
Cuts at: 0 373 460 768
Size: 373 87 308
Fragments arranged by size:
373 308 87
NarI GG'CG_CC
Cuts at: 0 558 768
Size: 558 210
NcoI C'CATG_G
Cuts at: 0 100 572 768
Size: 100 472 196
Fragments arranged by size:
472 196 100
PasI CC'CwG_GG
Cuts at: 0 553 768
Size: 553 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 735 768
Size: 735 33
PpuMI rG'GwC_Cy
Cuts at: 0 631 768
Size: 631 137
PstI C_TGCA'G
Cuts at: 0 659 768
Size: 659 109
SacII CC_GC'GG
Cuts at: 0 374 768
Size: 374 394
SanDI GG'GwC_CC
Cuts at: 0 631 768
Size: 631 137
SbfI CC_TGCA'GG
Cuts at: 0 659 768
Size: 659 109
SfcI C'TryA_G
Cuts at: 0 655 768
Size: 655 113
SfoI GGC'GCC
Cuts at: 0 559 768
Size: 559 209
SmaI CCC'GGG
Cuts at: 0 401 768
Size: 401 367
StuI AGG'CCT
Cuts at: 0 409 768
Size: 409 359
StyI C'CwwG_G
Cuts at: 0 100 572 768
Size: 100 472 196
Fragments arranged by size:
472 196 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 768
Size: 156 612
Tth111I GACn'n_nGTC
Cuts at: 0 264 768
Size: 264 504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 448 768
Size: 448 320
XmaI C'CCGG_G
Cuts at: 0 399 768
Size: 399 369
ZraI GAC'GTC
Cuts at: 0 681 768
Size: 681 87
Enzymes that do cut:
AatII AflIII AhdI AleI ApaLI AvaI BanI BanII
BbeI Bme1580I BmgBI BmrI BplI BsaHI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BssHII BstAPI
BtgI BtgZI BtsI DraIII EarI EcoO109I FspI HaeII
Hin4I KasI MluI MslI MspA1I NarI NcoI PasI
PflMI PpuMI PstI SacII SanDI SbfI SfcI SfoI
SmaI StuI StyI TaqII Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BsrBI BsrDI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV
FalI FseI FspAI HincII HindIII HpaI KpnI MfeI
MmeI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SapI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XbaI XhoI XmnI