(Linear) (Six-base) MAPSORT of: KIEE5257.seq  Check: 6142  from: 1  to: 624



KIEE5257.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:53  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      53     115     624 
   Size:         53      62     509

  Fragments arranged by size: 

                 509      62      53
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     475     624 
   Size:        475     149
 
AhdI GACnn_n'nnGTC

Cuts at:      0     132     624 
   Size:        132     492
 
ApoI r'AATT_y

Cuts at:      0     433     624 
   Size:        433     191
 
BanII G_rGCy'C

Cuts at:      0     555     624 
   Size:        555      69
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     624 
   Size:         83     541
 
BbvCI CC'TCA_GC

Cuts at:      0      30     624 
   Size:         30     594
 
BciVI GTATCCnnnnn_n'

Cuts at:      0       5     472     624 
   Size:          5     467     152

  Fragments arranged by size: 

                 467     152       5
 
BfrBI ATG'CAT

Cuts at:      0     493     624 
   Size:        493     131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     352     624 
   Size:        255      97     272

  Fragments arranged by size: 

                 272     255      97
 
Bpu10I CC'TnA_GC

Cuts at:      0      30     624 
   Size:         30     594
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253     624 
   Size:        253     371
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     153     162     624 
   Size:        153       9     462

  Fragments arranged by size: 

                 462     153       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     555     624 
   Size:        555      69
 
Bsp1286I G_dGCh'C

Cuts at:      0     555     624 
   Size:        555      69
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      53     115     624 
   Size:         53      62     509

  Fragments arranged by size: 

                 509      62      53
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     118     624 
   Size:        118     506
 
DraIII CAC_nnn'GTG

Cuts at:      0      25     624 
   Size:         25     599
 
EarI CTCTTCn'nnn_

Cuts at:      0     159     624 
   Size:        159     465
 
EcoICRI GAG'CTC

Cuts at:      0     553     624 
   Size:        553      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     352     475     624 
   Size:        255      97     123     149

  Fragments arranged by size: 

                 255     149     123      97
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     121     624 
   Size:        121     503
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     118     150     195     227     325     357     624 
   Size:        118      32      45      32      98      32     267

  Fragments arranged by size: 

                 267     118      98      45      32      32      32
 
MspA1I CmG'CkG

Cuts at:      0     233     296     624 
   Size:        233      63     328

  Fragments arranged by size: 

                 328     233      63
 
NsiI A_TGCA'T

Cuts at:      0     495     624 
   Size:        495     129
 
NspI r_CATG'y

Cuts at:      0     199     624 
   Size:        199     425
 
PfoI T'CCnGG_A

Cuts at:      0     329     624 
   Size:        329     295
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     304     336     624 
   Size:        304      32     288

  Fragments arranged by size: 

                 304     288      32
 
SacI G_AGCT'C

Cuts at:      0     555     624 
   Size:        555      69
 
ScaI AGT'ACT

Cuts at:      0     262     624 
   Size:        262     362
 
SmlI C'TyrA_G

Cuts at:      0     268     624 
   Size:        268     356
 
StyI C'CwwG_G

Cuts at:      0     117     556     624 
   Size:        117     439      68

  Fragments arranged by size: 

                 439     117      68
 
TatI w'GTAC_w

Cuts at:      0     260     624 
   Size:        260     364
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     182     214     624 
   Size:        182      32     410

  Fragments arranged by size: 

                 410     182      32
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     ApoI    BanII     BbsI    BbvCI    BciVI
    BfrBI     BpmI   Bpu10I    BpuEI    BseRI  BsiHKAI Bsp1286I    BspMI
    BstXI   DraIII     EarI  EcoICRI  Eco57MI    EcoNI    Hin4I   MspA1I
     NsiI     NspI     PfoI     PsrI     SacI     ScaI     SmlI     StyI
     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI