Restriction map for
pFN21AE5256
(Linear) (Six-base) MAPSORT of: KIEE5256.seq Check: 194 from: 1 to: 723
KIEE5256.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 676 723
Size: 604 72 47
Fragments arranged by size:
604 72 47
ApoI r'AATT_y
Cuts at: 0 391 406 723
Size: 391 15 317
Fragments arranged by size:
391 317 15
AseI AT'TA_AT
Cuts at: 0 355 723
Size: 355 368
AvaI C'yCGr_G
Cuts at: 0 551 723
Size: 551 172
BanI G'GyrC_C
Cuts at: 0 133 723
Size: 133 590
BanII G_rGCy'C
Cuts at: 0 68 339 723
Size: 68 271 384
Fragments arranged by size:
384 271 68
BbeI G_GCGC'C
Cuts at: 0 137 723
Size: 137 586
BbsI GAAGACnn'nnnn_
Cuts at: 0 512 723
Size: 512 211
BclI T'GATC_A
Cuts at: 0 566 723
Size: 566 157
BfrBI ATG'CAT
Cuts at: 0 382 723
Size: 382 341
BglII A'GATC_T
Cuts at: 0 279 723
Size: 279 444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 350 723
Size: 52 298 373
Fragments arranged by size:
373 298 52
BsaHI Gr'CG_yC
Cuts at: 0 134 723
Size: 134 589
BsiEI CG_ry'CG
Cuts at: 0 29 47 723
Size: 29 18 676
Fragments arranged by size:
676 29 18
BsmI GAATG_Cn'
Cuts at: 0 382 384 723
Size: 382 2 339
Fragments arranged by size:
382 339 2
Bsp1286I G_dGCh'C
Cuts at: 0 68 339 723
Size: 68 271 384
Fragments arranged by size:
384 271 68
BspHI T'CATG_A
Cuts at: 0 662 723
Size: 662 61
BsrBI CCG'CTC
Cuts at: 0 84 157 723
Size: 84 73 566
Fragments arranged by size:
566 84 73
BsrFI r'CCGG_y
Cuts at: 0 24 46 723
Size: 24 22 677
Fragments arranged by size:
677 24 22
BssSI C'ACGA_G
Cuts at: 0 213 723
Size: 213 510
BstYI r'GATC_y
Cuts at: 0 279 723
Size: 279 444
Bsu36I CC'TnA_GG
Cuts at: 0 530 723
Size: 530 193
EaeI y'GGCC_r
Cuts at: 0 26 44 723
Size: 26 18 679
Fragments arranged by size:
679 26 18
EagI C'GGCC_G
Cuts at: 0 26 44 723
Size: 26 18 679
Fragments arranged by size:
679 26 18
EarI CTCTTCn'nnn_
Cuts at: 0 433 476 723
Size: 433 43 247
Fragments arranged by size:
433 247 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 723
Size: 25 698
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 350 604 676 723
Size: 52 298 254 72 47
Fragments arranged by size:
298 254 72 52 47
EcoRI G'AATT_C
Cuts at: 0 391 406 723
Size: 391 15 317
Fragments arranged by size:
391 317 15
FspI TGC'GCA
Cuts at: 0 4 723
Size: 4 719
FspAI rTGC'GCAy
Cuts at: 0 4 723
Size: 4 719
HaeII r_GCGC'y
Cuts at: 0 137 723
Size: 137 586
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 551 583 723
Size: 551 32 140
Fragments arranged by size:
551 140 32
HincII GTy'rAC
Cuts at: 0 327 723
Size: 327 396
KasI G'GCGC_C
Cuts at: 0 133 723
Size: 133 590
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 310 723
Size: 197 113 413
Fragments arranged by size:
413 197 113
MslI CAynn'nnrTG
Cuts at: 0 634 723
Size: 634 89
NaeI GCC'GGC
Cuts at: 0 26 48 723
Size: 26 22 675
Fragments arranged by size:
675 26 22
NarI GG'CG_CC
Cuts at: 0 134 723
Size: 134 589
NgoMIV G'CCGG_C
Cuts at: 0 24 46 723
Size: 24 22 677
Fragments arranged by size:
677 24 22
NsiI A_TGCA'T
Cuts at: 0 384 723
Size: 384 339
NspI r_CATG'y
Cuts at: 0 182 723
Size: 182 541
SapI GCTCTTCn'nnn_
Cuts at: 0 433 723
Size: 433 290
SfoI GGC'GCC
Cuts at: 0 135 723
Size: 135 588
SmaI CCC'GGG
Cuts at: 0 553 723
Size: 553 170
SphI G_CATG'C
Cuts at: 0 182 723
Size: 182 541
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 723
Size: 426 32 265
Fragments arranged by size:
426 265 32
XmaI C'CCGG_G
Cuts at: 0 551 723
Size: 551 172
XmnI GAAnn'nnTTC
Cuts at: 0 382 723
Size: 382 341
Enzymes that do cut:
AcuI ApoI AseI AvaI BanI BanII BbeI BbsI
BclI BfrBI BglII BpmI BsaHI BsiEI BsmI Bsp1286I
BspHI BsrBI BsrFI BssSI BstYI Bsu36I EaeI EagI
EarI EciI Eco57MI EcoRI FspI FspAI HaeII Hin4I
HincII KasI MmeI MslI NaeI NarI NgoMIV NsiI
NspI SapI SfoI SmaI SphI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsiWI BsmBI BspEI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI HindIII HpaI KpnI MfeI
MluI MscI MspA1I NcoI NdeI NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI StyI SwaI TaqII TatI Tth111I
XbaI XcmI XhoI ZraI