Restriction map for
pFN21AE5255
(Linear) (Six-base) MAPSORT of: KIEE5255.seq Check: 5131 from: 1 to: 519
KIEE5255.orf
With 182 enzymes: SgfI *
December 5, 2014 13:00 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 63 519
Size: 63 456
ApaLI G'TGCA_C
Cuts at: 0 312 502 519
Size: 312 190 17
Fragments arranged by size:
312 190 17
BanI G'GyrC_C
Cuts at: 0 141 519
Size: 141 378
BanII G_rGCy'C
Cuts at: 0 46 519
Size: 46 473
BbeI G_GCGC'C
Cuts at: 0 145 519
Size: 145 374
BbsI GAAGACnn'nnnn_
Cuts at: 0 281 519
Size: 281 238
BclI T'GATC_A
Cuts at: 0 119 519
Size: 119 400
BlpI GC'TnA_GC
Cuts at: 0 404 519
Size: 404 115
Bme1580I G_kGCm'C
Cuts at: 0 316 506 519
Size: 316 190 13
Fragments arranged by size:
316 190 13
BmgBI CAC'GTC
Cuts at: 0 202 519
Size: 202 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 519
Size: 392 127
BsaHI Gr'CG_yC
Cuts at: 0 142 519
Size: 142 377
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 519
Size: 130 30 359
Fragments arranged by size:
359 130 30
BseYI C'CCAG_C
Cuts at: 0 213 392 519
Size: 213 179 127
Fragments arranged by size:
213 179 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 519
Size: 32 487
BsiHKAI G_wGCw'C
Cuts at: 0 316 506 519
Size: 316 190 13
Fragments arranged by size:
316 190 13
Bsp1286I G_dGCh'C
Cuts at: 0 46 316 506 519
Size: 46 270 190 13
Fragments arranged by size:
270 190 46 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4 519
Size: 4 515
BsrBI CCG'CTC
Cuts at: 0 164 519
Size: 164 355
BstEII G'GTnAC_C
Cuts at: 0 16 28 519
Size: 16 12 491
Fragments arranged by size:
491 16 12
BtgI C'CryG_G
Cuts at: 0 345 519
Size: 345 174
BtsI GCAGTG_nn'
Cuts at: 0 86 506 519
Size: 86 420 13
Fragments arranged by size:
420 86 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 519
Size: 392 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 55 519
Size: 55 464
EcoO109I rG'GnC_Cy
Cuts at: 0 350 519
Size: 350 169
EcoRV GAT'ATC
Cuts at: 0 380 519
Size: 380 139
HaeII r_GCGC'y
Cuts at: 0 145 519
Size: 145 374
KasI G'GCGC_C
Cuts at: 0 141 519
Size: 141 378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 519
Size: 282 237
MslI CAynn'nnrTG
Cuts at: 0 493 519
Size: 493 26
MspA1I CmG'CkG
Cuts at: 0 213 347 519
Size: 213 134 172
Fragments arranged by size:
213 172 134
NarI GG'CG_CC
Cuts at: 0 142 519
Size: 142 377
NspI r_CATG'y
Cuts at: 0 370 519
Size: 370 149
PpuMI rG'GwC_Cy
Cuts at: 0 350 519
Size: 350 169
SacII CC_GC'GG
Cuts at: 0 348 519
Size: 348 171
SanDI GG'GwC_CC
Cuts at: 0 350 519
Size: 350 169
SfoI GGC'GCC
Cuts at: 0 143 519
Size: 143 376
SphI G_CATG'C
Cuts at: 0 370 519
Size: 370 149
StuI AGG'CCT
Cuts at: 0 184 519
Size: 184 335
StyI C'CwwG_G
Cuts at: 0 257 519
Size: 257 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 229 519
Size: 229 290
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 191 223 414 446 519
Size: 65 32 94 32 191 32 73
Fragments arranged by size:
191 94 73 65 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 354 519
Size: 354 165
XbaI T'CTAG_A
Cuts at: 0 444 519
Size: 444 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 481 519
Size: 481 38
Enzymes that do cut:
AlwNI ApaLI BanI BanII BbeI BbsI BclI BlpI
Bme1580I BmgBI BpmI BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BstEII BtgI BtsI Eco57MI EcoNI
EcoO109I EcoRV HaeII KasI MmeI MslI MspA1I NarI
NspI PpuMI SacII SanDI SfoI SphI StuI StyI
TaqII TstI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI
BcgI BciVI BfrBI BglI BglII BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRI FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI SwaI TatI XhoI XmaI XmnI ZraI