Restriction map for
pFN21AE5254
(Linear) (Six-base) MAPSORT of: KIEE5254.seq Check: 7744 from: 1 to: 636
KIEE5254.orf
With 182 enzymes: SgfI *
January 29, 2014 11:53 ..
AflII C'TTAA_G
Cuts at: 0 88 636
Size: 88 548
AflIII A'CryG_T
Cuts at: 0 380 396 636
Size: 380 16 240
Fragments arranged by size:
380 240 16
AleI CACnn'nnGTG
Cuts at: 0 481 636
Size: 481 155
ApaI G_GGCC'C
Cuts at: 0 135 636
Size: 135 501
AvaI C'yCGr_G
Cuts at: 0 328 636
Size: 328 308
BanII G_rGCy'C
Cuts at: 0 27 135 636
Size: 27 108 501
Fragments arranged by size:
501 108 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 346 636
Size: 346 290
Bme1580I G_kGCm'C
Cuts at: 0 135 270 636
Size: 135 135 366
Fragments arranged by size:
366 135 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 636
Size: 163 473
Bpu10I CC'TnA_GC
Cuts at: 0 19 412 636
Size: 19 393 224
Fragments arranged by size:
393 224 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 265 636
Size: 265 371
BsaWI w'CCGG_w
Cuts at: 0 98 164 636
Size: 98 66 472
Fragments arranged by size:
472 98 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 636
Size: 175 30 431
Fragments arranged by size:
431 175 30
BseYI C'CCAG_C
Cuts at: 0 300 636
Size: 300 336
BsmI GAATG_Cn'
Cuts at: 0 310 636
Size: 310 326
Bsp1286I G_dGCh'C
Cuts at: 0 27 135 270 636
Size: 27 108 135 366
Fragments arranged by size:
366 135 108 27
BspEI T'CCGG_A
Cuts at: 0 98 164 636
Size: 98 66 472
Fragments arranged by size:
472 98 66
BspHI T'CATG_A
Cuts at: 0 149 636
Size: 149 487
BsrDI GCAATG_nn'
Cuts at: 0 585 636
Size: 585 51
BtsI GCAGTG_nn'
Cuts at: 0 46 476 636
Size: 46 430 160
Fragments arranged by size:
430 160 46
EaeI y'GGCC_r
Cuts at: 0 80 636
Size: 80 556
EarI CTCTTCn'nnn_
Cuts at: 0 166 636
Size: 166 470
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 636
Size: 163 473
EcoNI CCTnn'n_nnAGG
Cuts at: 0 568 636
Size: 568 68
EcoO109I rG'GnC_Cy
Cuts at: 0 132 459 636
Size: 132 327 177
Fragments arranged by size:
327 177 132
FspI TGC'GCA
Cuts at: 0 55 636
Size: 55 581
HaeII r_GCGC'y
Cuts at: 0 63 636
Size: 63 573
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 636
Size: 110 32 494
Fragments arranged by size:
494 110 32
HincII GTy'rAC
Cuts at: 0 42 636
Size: 42 594
MluI A'CGCG_T
Cuts at: 0 396 636
Size: 396 240
MslI CAynn'nnrTG
Cuts at: 0 481 636
Size: 481 155
MspA1I CmG'CkG
Cuts at: 0 471 636
Size: 471 165
NspI r_CATG'y
Cuts at: 0 384 636
Size: 384 252
PciI A'CATG_T
Cuts at: 0 380 636
Size: 380 256
PfoI T'CCnGG_A
Cuts at: 0 179 223 636
Size: 179 44 413
Fragments arranged by size:
413 179 44
PpuMI rG'GwC_Cy
Cuts at: 0 459 636
Size: 459 177
PspOMI G'GGCC_C
Cuts at: 0 131 636
Size: 131 505
PstI C_TGCA'G
Cuts at: 0 206 275 536 548 636
Size: 206 69 261 12 88
Fragments arranged by size:
261 206 88 69 12
SanDI GG'GwC_CC
Cuts at: 0 459 636
Size: 459 177
SfcI C'TryA_G
Cuts at: 0 202 271 425 532 544 636
Size: 202 69 154 107 12 92
Fragments arranged by size:
202 154 107 92 69 12
SmlI C'TyrA_G
Cuts at: 0 88 636
Size: 88 548
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 296 328 636
Size: 175 32 89 32 308
Fragments arranged by size:
308 175 89 32 32
Enzymes that do cut:
AflII AflIII AleI ApaI AvaI BanII BciVI Bme1580I
BpmI Bpu10I BsaI BsaWI BsaXI BseYI BsmI Bsp1286I
BspEI BspHI BsrDI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII MluI MslI MspA1I
NspI PciI PfoI PpuMI PspOMI PstI SanDI SfcI
SmlI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KasI KpnI MfeI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI