(Linear) (Six-base) MAPSORT of: KIEE5247.seq  Check: 2087  from: 1  to: 666



KIEE5247.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     562     666 
   Size:        562     104
 
ApoI r'AATT_y

Cuts at:      0      97     666 
   Size:         97     569
 
BanII G_rGCy'C

Cuts at:      0     270     666 
   Size:        270     396
 
BclI T'GATC_A

Cuts at:      0     368     666 
   Size:        368     298
 
BlpI GC'TnA_GC

Cuts at:      0     457     666 
   Size:        457     209
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     229     528     666 
   Size:        229     299     138

  Fragments arranged by size: 

                 299     229     138
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     498     510     666 
   Size:         98     400      12     156

  Fragments arranged by size: 

                 400     156      98      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     517     666 
   Size:        517     149
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     666 
   Size:        217     449
 
Bsp1286I G_dGCh'C

Cuts at:      0     270     666 
   Size:        270     396
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     578     666 
   Size:        578      88
 
BsrDI GCAATG_nn'

Cuts at:      0     221     666 
   Size:        221     445
 
Bsu36I CC'TnA_GG

Cuts at:      0     341     666 
   Size:        341     325
 
BtgI C'CryG_G

Cuts at:      0     426     620     666 
   Size:        426     194      46

  Fragments arranged by size: 

                 426     194      46
 
DraIII CAC_nnn'GTG

Cuts at:      0      59     666 
   Size:         59     607
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     666 
   Size:         86     580
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     498     510     562     666 
   Size:         98     400      12      52     104

  Fragments arranged by size: 

                 400     104      98      52      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0     546     666 
   Size:        546     120
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     381     457     666 
   Size:        381      76     209

  Fragments arranged by size: 

                 381     209      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     616     648     666 
   Size:        616      32      18

  Fragments arranged by size: 

                 616      32      18
 
HindIII A'AGCT_T

Cuts at:      0     358     666 
   Size:        358     308
 
MspA1I CmG'CkG

Cuts at:      0      76     666 
   Size:         76     590
 
NcoI C'CATG_G

Cuts at:      0     426     620     666 
   Size:        426     194      46

  Fragments arranged by size: 

                 426     194      46
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      59     666 
   Size:         59     607
 
PshAI GACnn'nnGTC

Cuts at:      0     515     666 
   Size:        515     151
 
PsiI TTA'TAA

Cuts at:      0     103     666 
   Size:        103     563
 
PstI C_TGCA'G

Cuts at:      0      75     114     666 
   Size:         75      39     552

  Fragments arranged by size: 

                 552      75      39
 
PvuII CAG'CTG

Cuts at:      0      76     666 
   Size:         76     590
 
SfcI C'TryA_G

Cuts at:      0      71     110     592     666 
   Size:         71      39     482      74

  Fragments arranged by size: 

                 482      74      71      39
 
StyI C'CwwG_G

Cuts at:      0     426     607     620     666 
   Size:        426     181      13      46

  Fragments arranged by size: 

                 426     181      46      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     295     666 
   Size:        295     371
 
XmnI GAAnn'nnTTC

Cuts at:      0     655     666 
   Size:        655      11
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BclI     BlpI     BplI     BpmI    BseRI
     BsgI Bsp1286I    BspMI    BsrDI   Bsu36I     BtgI   DraIII     EarI
  Eco57MI EcoO109I     FalI    Hin4I  HindIII   MspA1I     NcoI    PflMI
    PshAI     PsiI     PstI    PvuII     SfcI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI