Restriction map for
pFN21AE5239
(Linear) (Six-base) MAPSORT of: KIEE5239.seq Check: 7391 from: 1 to: 768
KIEE5239.orf
With 182 enzymes: SgfI *
January 29, 2014 11:52 ..
AatII G_ACGT'C
Cuts at: 0 289 768
Size: 289 479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 768
Size: 250 518
AflIII A'CryG_T
Cuts at: 0 665 768
Size: 665 103
AleI CACnn'nnGTG
Cuts at: 0 712 768
Size: 712 56
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 768
Size: 190 578
AlwNI CAG_nnn'CTG
Cuts at: 0 93 345 768
Size: 93 252 423
Fragments arranged by size:
423 252 93
ApoI r'AATT_y
Cuts at: 0 563 768
Size: 563 205
BamHI G'GATC_C
Cuts at: 0 515 768
Size: 515 253
BanII G_rGCy'C
Cuts at: 0 370 768
Size: 370 398
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 598 768
Size: 18 580 170
Fragments arranged by size:
580 170 18
BbvCI CC'TCA_GC
Cuts at: 0 163 768
Size: 163 605
BglI GCCn_nnn'nGGC
Cuts at: 0 194 374 768
Size: 194 180 394
Fragments arranged by size:
394 194 180
Bme1580I G_kGCm'C
Cuts at: 0 80 673 768
Size: 80 593 95
Fragments arranged by size:
593 95 80
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 51 768
Size: 20 31 717
Fragments arranged by size:
717 31 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 768
Size: 257 511
Bpu10I CC'TnA_GC
Cuts at: 0 163 768
Size: 163 605
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 768
Size: 144 624
BsaHI Gr'CG_yC
Cuts at: 0 286 768
Size: 286 482
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 254 768
Size: 254 514
BseYI C'CCAG_C
Cuts at: 0 338 768
Size: 338 430
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 522 768
Size: 382 140 246
Fragments arranged by size:
382 246 140
BsmI GAATG_Cn'
Cuts at: 0 197 768
Size: 197 571
BsmBI CGTCTCn'nnnn_
Cuts at: 0 388 768
Size: 388 380
Bsp1286I G_dGCh'C
Cuts at: 0 80 370 673 768
Size: 80 290 303 95
Fragments arranged by size:
303 290 95 80
BsrBI CCG'CTC
Cuts at: 0 216 768
Size: 216 552
BsrDI GCAATG_nn'
Cuts at: 0 174 768
Size: 174 594
BstAPI GCAn_nnn'nTGC
Cuts at: 0 668 768
Size: 668 100
BstXI CCAn_nnnn'nTGG
Cuts at: 0 550 768
Size: 550 218
BstYI r'GATC_y
Cuts at: 0 515 626 768
Size: 515 111 142
Fragments arranged by size:
515 142 111
BtgI C'CryG_G
Cuts at: 0 50 741 768
Size: 50 691 27
Fragments arranged by size:
691 50 27
BtsI GCAGTG_nn'
Cuts at: 0 476 768
Size: 476 292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 120 155 768
Size: 120 35 613
Fragments arranged by size:
613 120 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 325 768
Size: 325 443
EaeI y'GGCC_r
Cuts at: 0 375 768
Size: 375 393
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 257 768
Size: 250 7 511
Fragments arranged by size:
511 250 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 239 768
Size: 239 529
EcoO109I rG'GnC_Cy
Cuts at: 0 15 186 768
Size: 15 171 582
Fragments arranged by size:
582 171 15
HincII GTy'rAC
Cuts at: 0 729 768
Size: 729 39
HindIII A'AGCT_T
Cuts at: 0 147 768
Size: 147 621
HpaI GTT'AAC
Cuts at: 0 729 768
Size: 729 39
MscI TGG'CCA
Cuts at: 0 377 768
Size: 377 391
MslI CAynn'nnrTG
Cuts at: 0 712 768
Size: 712 56
MspA1I CmG'CkG
Cuts at: 0 342 768
Size: 342 426
NcoI C'CATG_G
Cuts at: 0 50 768
Size: 50 718
NspI r_CATG'y
Cuts at: 0 735 768
Size: 735 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 345 768
Size: 51 294 423
Fragments arranged by size:
423 294 51
PfoI T'CCnGG_A
Cuts at: 0 218 768
Size: 218 550
PstI C_TGCA'G
Cuts at: 0 483 768
Size: 483 285
PvuII CAG'CTG
Cuts at: 0 342 768
Size: 342 426
SexAI A'CCwGG_T
Cuts at: 0 521 768
Size: 521 247
SfcI C'TryA_G
Cuts at: 0 479 748 768
Size: 479 269 20
Fragments arranged by size:
479 269 20
SmlI C'TyrA_G
Cuts at: 0 123 768
Size: 123 645
StuI AGG'CCT
Cuts at: 0 68 768
Size: 68 700
StyI C'CwwG_G
Cuts at: 0 50 261 528 768
Size: 50 211 267 240
Fragments arranged by size:
267 240 211 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 768
Size: 556 212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 526 768
Size: 526 242
XmnI GAAnn'nnTTC
Cuts at: 0 209 768
Size: 209 559
ZraI GAC'GTC
Cuts at: 0 287 768
Size: 287 481
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI ApoI BamHI
BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaHI BseRI BseYI BsgI BsmI BsmBI Bsp1286I
BsrBI BsrDI BstAPI BstXI BstYI BtgI BtsI CspCI
DrdI EaeI Eco57MI EcoNI EcoO109I HincII HindIII HpaI
MscI MslI MspA1I NcoI NspI PflMI PfoI PstI
PvuII SexAI SfcI SmlI StuI StyI TaqII XcmI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BspEI BspHI BspMI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI DraI DraIII EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XhoI XmaI