(Linear) (Six-base) MAPSORT of: KIEE5238.seq  Check: 3397  from: 1  to: 777



KIEE5238.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     500     777 
   Size:        500     277
 
ApoI r'AATT_y

Cuts at:      0      21      51     139     195     777 
   Size:         21      30      88      56     582

  Fragments arranged by size: 

                 582      88      56      30      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     370     777 
   Size:        370     407
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     628     689     777 
   Size:        628      61      88

  Fragments arranged by size: 

                 628      88      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     733     777 
   Size:        733      44
 
BsaBI GATnn'nnATC

Cuts at:      0     476     777 
   Size:        476     301
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      52     777 
   Size:         52     725
 
BsrDI GCAATG_nn'

Cuts at:      0     643     777 
   Size:        643     134
 
EcoRI G'AATT_C

Cuts at:      0      21      51     777 
   Size:         21      30     726

  Fragments arranged by size: 

                 726      30      21
 
HindIII A'AGCT_T

Cuts at:      0     334     777 
   Size:        334     443
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     558     777 
   Size:        558     219
 
MslI CAynn'nnrTG

Cuts at:      0     482     726     777 
   Size:        482     244      51

  Fragments arranged by size: 

                 482     244      51
 
ScaI AGT'ACT

Cuts at:      0      12     777 
   Size:         12     765
 
SfcI C'TryA_G

Cuts at:      0     287     777 
   Size:        287     490
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     754     777 
   Size:        754      23
 
TatI w'GTAC_w

Cuts at:      0      10     351     764     777 
   Size:         10     341     413      13

  Fragments arranged by size: 

                 413     341      13      10
 

 Enzymes that do cut:

     AlfI     ApoI     BbsI    BciVI     BmrI    BsaBI     BsgI    BsrDI
    EcoRI  HindIII     MmeI     MslI     ScaI     SfcI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI