(Linear) (Six-base) MAPSORT of: KIEE5235.seq  Check: 2651  from: 1  to: 588



KIEE5235.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     394     454     588 
   Size:          1     393      60     134

  Fragments arranged by size: 

                 393     134      60       1
 
AflIII A'CryG_T

Cuts at:      0     353     588 
   Size:        353     235
 
AvaI C'yCGr_G

Cuts at:      0     176     588 
   Size:        176     412
 
BanII G_rGCy'C

Cuts at:      0     183     588 
   Size:        183     405
 
BbvCI CC'TCA_GC

Cuts at:      0     476     588 
   Size:        476     112
 
BclI T'GATC_A

Cuts at:      0     146     588 
   Size:        146     442
 
BglI GCCn_nnn'nGGC

Cuts at:      0     215     588 
   Size:        215     373
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     588 
   Size:        514      74
 
Bpu10I CC'TnA_GC

Cuts at:      0      10     476     588 
   Size:         10     466     112

  Fragments arranged by size: 

                 466     112      10
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      76     571     588 
   Size:         76     495      17

  Fragments arranged by size: 

                 495      76      17
 
BsaWI w'CCGG_w

Cuts at:      0     122     588 
   Size:        122     466
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     303     559     588 
   Size:        303     256      29

  Fragments arranged by size: 

                 303     256      29
 
BsiEI CG_ry'CG

Cuts at:      0     348     588 
   Size:        348     240
 
BsmI GAATG_Cn'

Cuts at:      0     269     588 
   Size:        269     319
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     588 
   Size:        183     405
 
BspEI T'CCGG_A

Cuts at:      0     122     588 
   Size:        122     466
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     476     588 
   Size:        476     112
 
BsrFI r'CCGG_y

Cuts at:      0     308     588 
   Size:        308     280
 
BstYI r'GATC_y

Cuts at:      0     119     588 
   Size:        119     469
 
ClaI AT'CG_AT

Cuts at:      0     344     588 
   Size:        344     244
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     515     569     588 
   Size:         16     499      54      19

  Fragments arranged by size: 

                 499      54      19      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1     394     454     514     588 
   Size:          1     393      60      60      74

  Fragments arranged by size: 

                 393      74      60      60       1
 
HaeII r_GCGC'y

Cuts at:      0     516     528     588 
   Size:        516      12      60

  Fragments arranged by size: 

                 516      60      12
 
MslI CAynn'nnrTG

Cuts at:      0     283     588 
   Size:        283     305
 
MspA1I CmG'CkG

Cuts at:      0      47     588 
   Size:         47     541
 
NaeI GCC'GGC

Cuts at:      0     310     588 
   Size:        310     278
 
NgoMIV G'CCGG_C

Cuts at:      0     308     588 
   Size:        308     280
 
NspI r_CATG'y

Cuts at:      0     357     588 
   Size:        357     231
 
PciI A'CATG_T

Cuts at:      0     353     588 
   Size:        353     235
 
PvuI CG_AT'CG

Cuts at:      0     348     588 
   Size:        348     240
 
SapI GCTCTTCn'nnn_

Cuts at:      0     515     588 
   Size:        515      73
 
SmaI CCC'GGG

Cuts at:      0     178     588 
   Size:        178     410
 
StyI C'CwwG_G

Cuts at:      0     200     497     566     588 
   Size:        200     297      69      22

  Fragments arranged by size: 

                 297     200      69      22
 
XmaI C'CCGG_G

Cuts at:      0     176     588 
   Size:        176     412
 
XmnI GAAnn'nnTTC

Cuts at:      0     391     588 
   Size:        391     197
 

 Enzymes that do cut:

     AcuI   AflIII     AvaI    BanII    BbvCI     BclI     BglI     BpmI
   Bpu10I     BsaI    BsaWI    BseRI    BsiEI     BsmI Bsp1286I    BspEI
    BspMI    BsrFI    BstYI     ClaI     EarI  Eco57MI    HaeII     MslI
   MspA1I     NaeI   NgoMIV     NspI     PciI     PvuI     SapI     SmaI
     StyI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI     BcgI    BciVI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI    BspHI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     ZraI