(Linear) (Six-base) MAPSORT of: KIEE5234.seq  Check: 3839  from: 1  to: 633



KIEE5234.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:52  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     594     624     633 
   Size:        594      30       9

  Fragments arranged by size: 

                 594      30       9
 
AvaI C'yCGr_G

Cuts at:      0      83     307     633 
   Size:         83     224     326

  Fragments arranged by size: 

                 326     224      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     126     633 
   Size:        126     507
 
BglI GCCn_nnn'nGGC

Cuts at:      0     257     633 
   Size:        257     376
 
BlpI GC'TnA_GC

Cuts at:      0     421     633 
   Size:        421     212
 
Bme1580I G_kGCm'C

Cuts at:      0       7     633 
   Size:          7     626
 
BmgBI CAC'GTC

Cuts at:      0     270     633 
   Size:        270     363
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     633 
   Size:        115     518
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      48     633 
   Size:         48     585
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      74     209     633 
   Size:         74     135     424

  Fragments arranged by size: 

                 424     135      74
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     142     633 
   Size:        112      30     491

  Fragments arranged by size: 

                 491     112      30
 
BseYI C'CCAG_C

Cuts at:      0     324     633 
   Size:        324     309
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     633 
   Size:          7     626
 
BsrBI CCG'CTC

Cuts at:      0     147     633 
   Size:        147     486
 
BsrFI r'CCGG_y

Cuts at:      0     290     633 
   Size:        290     343
 
BssSI C'ACGA_G

Cuts at:      0     523     633 
   Size:        523     110
 
BstBI TT'CG_AA

Cuts at:      0      78     633 
   Size:         78     555
 
BstYI r'GATC_y

Cuts at:      0     440     633 
   Size:        440     193
 
Bsu36I CC'TnA_GG

Cuts at:      0     583     633 
   Size:        583      50
 
EaeI y'GGCC_r

Cuts at:      0     196     633 
   Size:        196     437
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     465     633 
   Size:        465     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     633 
   Size:        115     518
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     526     633 
   Size:        526     107
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     633 
   Size:         89     544
 
HaeII r_GCGC'y

Cuts at:      0     421     633 
   Size:        421     212
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     425     457     548     580     633 
   Size:        425      32      91      32      53

  Fragments arranged by size: 

                 425      91      53      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     633 
   Size:        122     511
 
MscI TGG'CCA

Cuts at:      0     198     633 
   Size:        198     435
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     225     633 
   Size:        225     408
 
PfoI T'CCnGG_A

Cuts at:      0      52     633 
   Size:         52     581
 
PpuMI rG'GwC_Cy

Cuts at:      0      89     633 
   Size:         89     544
 
PspXI vC'TCGA_Gb

Cuts at:      0      83     633 
   Size:         83     550
 
SanDI GG'GwC_CC

Cuts at:      0      89     633 
   Size:         89     544
 
SmlI C'TyrA_G

Cuts at:      0      63      83     633 
   Size:         63      20     550

  Fragments arranged by size: 

                 550      63      20
 
StuI AGG'CCT

Cuts at:      0     203     313     633 
   Size:        203     110     320

  Fragments arranged by size: 

                 320     203     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     331     633 
   Size:        331     302
 
Tth111I GACn'n_nGTC

Cuts at:      0     190     633 
   Size:        190     443
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     486     633 
   Size:        486     147
 
XhoI C'TCGA_G

Cuts at:      0      83     633 
   Size:         83     550
 

 Enzymes that do cut:

    AlwNI     AvaI     BbsI     BglI     BlpI Bme1580I    BmgBI     BpmI
    BpuEI     BsaI    BseRI    BseYI Bsp1286I    BsrBI    BsrFI    BssSI
    BstBI    BstYI   Bsu36I     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII    Hin4I     MmeI     MscI    PflMI     PfoI    PpuMI    PspXI
    SanDI     SmlI     StuI    TaqII  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrGI   BssHII   BstAPI   BstEII    BstXI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI
     TstI     XbaI     XmaI     XmnI     ZraI