Restriction map for
pFN21AE5232
(Linear) (Six-base) MAPSORT of: KIEE5232.seq Check: 4584 from: 1 to: 753
KIEE5232.orf
With 182 enzymes: SgfI *
January 29, 2014 11:52 ..
AccI GT'mk_AC
Cuts at: 0 572 753
Size: 572 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 454 753
Size: 207 247 299
Fragments arranged by size:
299 247 207
AleI CACnn'nnGTG
Cuts at: 0 365 753
Size: 365 388
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 571 603 753
Size: 571 32 150
Fragments arranged by size:
571 150 32
AvaI C'yCGr_G
Cuts at: 0 325 753
Size: 325 428
BanI G'GyrC_C
Cuts at: 0 723 753
Size: 723 30
BanII G_rGCy'C
Cuts at: 0 326 671 753
Size: 326 345 82
Fragments arranged by size:
345 326 82
BbvCI CC'TCA_GC
Cuts at: 0 650 753
Size: 650 103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 717 753
Size: 717 36
Bpu10I CC'TnA_GC
Cuts at: 0 650 753
Size: 650 103
BsaHI Gr'CG_yC
Cuts at: 0 464 753
Size: 464 289
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 753
Size: 571 30 152
Fragments arranged by size:
571 152 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 433 453 517 520 592 673 676
Size: 430 3 20 64 3 72 81 3
Cuts at: 676 679 709 753
Size: 3 30 44
Fragments arranged by size:
430 81 72 64 44 30 20 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 98 753
Size: 98 655
BsiEI CG_ry'CG
Cuts at: 0 569 753
Size: 569 184
BsiHKAI G_wGCw'C
Cuts at: 0 92 671 753
Size: 92 579 82
Fragments arranged by size:
579 92 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 133 299 753
Size: 133 166 454
Fragments arranged by size:
454 166 133
Bsp1286I G_dGCh'C
Cuts at: 0 92 326 671 753
Size: 92 234 345 82
Fragments arranged by size:
345 234 92 82
BsrBI CCG'CTC
Cuts at: 0 32 753
Size: 32 721
BsrFI r'CCGG_y
Cuts at: 0 75 753
Size: 75 678
BsrGI T'GTAC_A
Cuts at: 0 23 753
Size: 23 730
BssSI C'ACGA_G
Cuts at: 0 136 592 753
Size: 136 456 161
Fragments arranged by size:
456 161 136
BstXI CCAn_nnnn'nTGG
Cuts at: 0 367 753
Size: 367 386
BtgI C'CryG_G
Cuts at: 0 257 753
Size: 257 496
EaeI y'GGCC_r
Cuts at: 0 73 316 566 753
Size: 73 243 250 187
Fragments arranged by size:
250 243 187 73
EagI C'GGCC_G
Cuts at: 0 566 753
Size: 566 187
EarI CTCTTCn'nnn_
Cuts at: 0 430 753
Size: 430 323
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 532 544 749 753
Size: 526 6 12 205 4
Fragments arranged by size:
526 205 12 6 4
EcoICRI GAG'CTC
Cuts at: 0 669 753
Size: 669 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 454 753
Size: 207 247 299
Fragments arranged by size:
299 247 207
EcoO109I rG'GnC_Cy
Cuts at: 0 36 131 450 753
Size: 36 95 319 303
Fragments arranged by size:
319 303 95 36
HaeII r_GCGC'y
Cuts at: 0 617 753
Size: 617 136
HincII GTy'rAC
Cuts at: 0 573 753
Size: 573 180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 753
Size: 296 457
MslI CAynn'nnrTG
Cuts at: 0 19 365 753
Size: 19 346 388
Fragments arranged by size:
388 346 19
MspA1I CmG'CkG
Cuts at: 0 302 432 753
Size: 302 130 321
Fragments arranged by size:
321 302 130
NaeI GCC'GGC
Cuts at: 0 77 753
Size: 77 676
NgoMIV G'CCGG_C
Cuts at: 0 75 753
Size: 75 678
NruI TCG'CGA
Cuts at: 0 743 753
Size: 743 10
NspI r_CATG'y
Cuts at: 0 18 718 753
Size: 18 700 35
Fragments arranged by size:
700 35 18
PfoI T'CCnGG_A
Cuts at: 0 671 753
Size: 671 82
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 571 603 753
Size: 571 32 150
Fragments arranged by size:
571 150 32
PpuMI rG'GwC_Cy
Cuts at: 0 36 131 450 753
Size: 36 95 319 303
Fragments arranged by size:
319 303 95 36
PshAI GACnn'nnGTC
Cuts at: 0 568 753
Size: 568 185
SacI G_AGCT'C
Cuts at: 0 671 753
Size: 671 82
SalI G'TCGA_C
Cuts at: 0 571 753
Size: 571 182
SanDI GG'GwC_CC
Cuts at: 0 131 450 753
Size: 131 319 303
Fragments arranged by size:
319 303 131
SexAI A'CCwGG_T
Cuts at: 0 38 113 753
Size: 38 75 640
Fragments arranged by size:
640 75 38
SphI G_CATG'C
Cuts at: 0 18 718 753
Size: 18 700 35
Fragments arranged by size:
700 35 18
StuI AGG'CCT
Cuts at: 0 268 700 753
Size: 268 432 53
Fragments arranged by size:
432 268 53
StyI C'CwwG_G
Cuts at: 0 313 479 753
Size: 313 166 274
Fragments arranged by size:
313 274 166
TatI w'GTAC_w
Cuts at: 0 23 753
Size: 23 730
Enzymes that do cut:
AccI AcuI AleI AloI AvaI BanI BanII BbvCI
BplI Bpu10I BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BsrBI BsrFI BsrGI BssSI BstXI BtgI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
HincII MmeI MslI MspA1I NaeI NgoMIV NruI NspI
PfoI PpiI PpuMI PshAI SacI SalI SanDI SexAI
SphI StuI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiWI BsmI BspEI BspHI BspMI BsrDI BssHII BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NsiI PacI PasI PciI PflMI PmeI PmlI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI