Restriction map for
pFN21AE5227
(Linear) (Six-base) MAPSORT of: KIEE5227.seq Check: 7075 from: 1 to: 582
KIEE5227.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 582
Size: 311 271
AleI CACnn'nnGTG
Cuts at: 0 103 582
Size: 103 479
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 508 540 582
Size: 508 32 42
Fragments arranged by size:
508 42 32
AlwNI CAG_nnn'CTG
Cuts at: 0 309 434 582
Size: 309 125 148
Fragments arranged by size:
309 148 125
ApaI G_GGCC'C
Cuts at: 0 200 543 582
Size: 200 343 39
Fragments arranged by size:
343 200 39
AvaI C'yCGr_G
Cuts at: 0 408 582
Size: 408 174
BanI G'GyrC_C
Cuts at: 0 423 582
Size: 423 159
BanII G_rGCy'C
Cuts at: 0 200 248 543 582
Size: 200 48 295 39
Fragments arranged by size:
295 200 48 39
Bme1580I G_kGCm'C
Cuts at: 0 200 543 582
Size: 200 343 39
Fragments arranged by size:
343 200 39
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 295 568 582
Size: 79 216 273 14
Fragments arranged by size:
273 216 79 14
BsaI GGTCTCn'nnnn_
Cuts at: 0 301 582
Size: 301 281
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 508 538 582
Size: 508 30 44
Fragments arranged by size:
508 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 582
Size: 76 506
BseYI C'CCAG_C
Cuts at: 0 449 582
Size: 449 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 582
Size: 330 252
BsiWI C'GTAC_G
Cuts at: 0 488 582
Size: 488 94
Bsp1286I G_dGCh'C
Cuts at: 0 200 248 543 582
Size: 200 48 295 39
Fragments arranged by size:
295 200 48 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 582
Size: 131 451
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 445 582
Size: 57 388 137
Fragments arranged by size:
388 137 57
Bsu36I CC'TnA_GG
Cuts at: 0 503 582
Size: 503 79
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 514 582
Size: 514 68
EaeI y'GGCC_r
Cuts at: 0 311 582
Size: 311 271
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 523 582
Size: 523 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 295 311 568 582
Size: 79 216 16 257 14
Fragments arranged by size:
257 216 79 16 14
EcoO109I rG'GnC_Cy
Cuts at: 0 196 369 453 540 582
Size: 196 173 84 87 42
Fragments arranged by size:
196 173 87 84 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 582
Size: 313 32 237
Fragments arranged by size:
313 237 32
MslI CAynn'nnrTG
Cuts at: 0 103 582
Size: 103 479
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 582
Size: 309 273
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 508 540 582
Size: 508 32 42
Fragments arranged by size:
508 42 32
PpuMI rG'GwC_Cy
Cuts at: 0 369 453 582
Size: 369 84 129
Fragments arranged by size:
369 129 84
PshAI GACnn'nnGTC
Cuts at: 0 218 582
Size: 218 364
PspOMI G'GGCC_C
Cuts at: 0 196 539 582
Size: 196 343 43
Fragments arranged by size:
343 196 43
PstI C_TGCA'G
Cuts at: 0 557 582
Size: 557 25
SanDI GG'GwC_CC
Cuts at: 0 369 453 582
Size: 369 84 129
Fragments arranged by size:
369 129 84
ScaI AGT'ACT
Cuts at: 0 562 582
Size: 562 20
SfcI C'TryA_G
Cuts at: 0 553 582
Size: 553 29
StyI C'CwwG_G
Cuts at: 0 237 582
Size: 237 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 441 582
Size: 441 141
TatI w'GTAC_w
Cuts at: 0 560 582
Size: 560 22
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI AvaI BanI BanII
Bme1580I BpmI BsaI BsaXI BseRI BseYI BsgI BsiWI
Bsp1286I BspMI BstXI Bsu36I BtgZI EaeI EciI Eco57MI
EcoO109I Hin4I MslI PflMI PpiI PpuMI PshAI PspOMI
PstI SanDI ScaI SfcI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI
EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI