Restriction map for
pFN21AE5216
(Linear) (Six-base) MAPSORT of: KIEE5216.seq Check: 233 from: 1 to: 597
KIEE5216.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 423 597
Size: 423 174
AccI GT'mk_AC
Cuts at: 0 23 597
Size: 23 574
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 597
Size: 38 559
AflIII A'CryG_T
Cuts at: 0 154 597
Size: 154 443
AlwNI CAG_nnn'CTG
Cuts at: 0 480 597
Size: 480 117
AvaI C'yCGr_G
Cuts at: 0 287 597
Size: 287 310
BanII G_rGCy'C
Cuts at: 0 44 320 331 508 597
Size: 44 276 11 177 89
Fragments arranged by size:
276 177 89 44 11
BbvCI CC'TCA_GC
Cuts at: 0 306 597
Size: 306 291
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 85 139 173 597
Size: 51 34 54 34 424
Fragments arranged by size:
424 54 51 34 34
BmgBI CAC'GTC
Cuts at: 0 155 237 597
Size: 155 82 360
Fragments arranged by size:
360 155 82
BmrI ACTGGGnnnn_n'
Cuts at: 0 346 511 597
Size: 346 165 86
Fragments arranged by size:
346 165 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 597
Size: 541 56
Bpu10I CC'TnA_GC
Cuts at: 0 306 597
Size: 306 291
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 597
Size: 379 218
BsaWI w'CCGG_w
Cuts at: 0 363 597
Size: 363 234
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 16 35 46 597
Size: 5 11 19 11 551
Fragments arranged by size:
551 19 11 11 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 169 597
Size: 169 428
BseYI C'CCAG_C
Cuts at: 0 144 344 597
Size: 144 200 253
Fragments arranged by size:
253 200 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 438 597
Size: 438 159
BsiHKAI G_wGCw'C
Cuts at: 0 296 320 371 597
Size: 296 24 51 226
Fragments arranged by size:
296 226 51 24
BsmI GAATG_Cn'
Cuts at: 0 267 597
Size: 267 330
BsmBI CGTCTCn'nnnn_
Cuts at: 0 52 145 597
Size: 52 93 452
Fragments arranged by size:
452 93 52
Bsp1286I G_dGCh'C
Cuts at: 0 44 296 320 331 371 508 597
Size: 44 252 24 11 40 137 89
Fragments arranged by size:
252 137 89 44 40 24 11
BspEI T'CCGG_A
Cuts at: 0 363 597
Size: 363 234
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 423 597
Size: 345 78 174
Fragments arranged by size:
345 174 78
BsrBI CCG'CTC
Cuts at: 0 87 256 597
Size: 87 169 341
Fragments arranged by size:
341 169 87
BstEII G'GTnAC_C
Cuts at: 0 133 597
Size: 133 464
BstXI CCAn_nnnn'nTGG
Cuts at: 0 541 597
Size: 541 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 597
Size: 70 527
EcoICRI GAG'CTC
Cuts at: 0 318 597
Size: 318 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 541 597
Size: 38 503 56
Fragments arranged by size:
503 56 38
EcoO109I rG'GnC_Cy
Cuts at: 0 567 597
Size: 567 30
FspI TGC'GCA
Cuts at: 0 574 597
Size: 574 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 16 37 48 85 117 597
Size: 5 11 21 11 37 32 480
Fragments arranged by size:
480 37 32 21 11 11 5
HincII GTy'rAC
Cuts at: 0 24 597
Size: 24 573
MspA1I CmG'CkG
Cuts at: 0 195 300 537 597
Size: 195 105 237 60
Fragments arranged by size:
237 195 105 60
NspI r_CATG'y
Cuts at: 0 144 560 597
Size: 144 416 37
Fragments arranged by size:
416 144 37
PasI CC'CwG_GG
Cuts at: 0 324 405 597
Size: 324 81 192
Fragments arranged by size:
324 192 81
PstI C_TGCA'G
Cuts at: 0 74 387 584 597
Size: 74 313 197 13
Fragments arranged by size:
313 197 74 13
PvuII CAG'CTG
Cuts at: 0 300 537 597
Size: 300 237 60
Fragments arranged by size:
300 237 60
SacI G_AGCT'C
Cuts at: 0 320 597
Size: 320 277
SalI G'TCGA_C
Cuts at: 0 22 597
Size: 22 575
SfcI C'TryA_G
Cuts at: 0 70 383 580 589 597
Size: 70 313 197 9 8
Fragments arranged by size:
313 197 70 9 8
SmlI C'TyrA_G
Cuts at: 0 394 597
Size: 394 203
SphI G_CATG'C
Cuts at: 0 144 597
Size: 144 453
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI AvaI BanII BbvCI
BcgI BmgBI BmrI BpmI Bpu10I BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrBI BstEII BstXI EciI EcoICRI Eco57MI EcoO109I
FspI Hin4I HincII MspA1I NspI PasI PstI PvuII
SacI SalI SfcI SmlI SphI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmtI BplI BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EcoNI EcoRI EcoRV FalI FseI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI