Restriction map for
pFN21AE5214
(Linear) (Six-base) MAPSORT of: KIEE5214.seq Check: 4270 from: 1 to: 645
KIEE5214.orf
With 182 enzymes: SgfI *
January 29, 2014 11:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 289 607 645
Size: 18 271 318 38
Fragments arranged by size:
318 271 38 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 26 645
Size: 26 619
AlwNI CAG_nnn'CTG
Cuts at: 0 103 570 645
Size: 103 467 75
Fragments arranged by size:
467 103 75
BanI G'GyrC_C
Cuts at: 0 69 487 637 645
Size: 69 418 150 8
Fragments arranged by size:
418 150 69 8
BanII G_rGCy'C
Cuts at: 0 50 452 645
Size: 50 402 193
Fragments arranged by size:
402 193 50
BbeI G_GCGC'C
Cuts at: 0 641 645
Size: 641 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 595 645
Size: 595 50
BglI GCCn_nnn'nGGC
Cuts at: 0 410 645
Size: 410 235
BlpI GC'TnA_GC
Cuts at: 0 571 645
Size: 571 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 480 645
Size: 480 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 295 645
Size: 226 69 350
Fragments arranged by size:
350 226 69
Bpu10I CC'TnA_GC
Cuts at: 0 455 645
Size: 455 190
BsaHI Gr'CG_yC
Cuts at: 0 134 638 645
Size: 134 504 7
Fragments arranged by size:
504 134 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 564 610 613 645
Size: 244 320 46 3 32
Fragments arranged by size:
320 244 46 32 3
BseYI C'CCAG_C
Cuts at: 0 313 645
Size: 313 332
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 370 645
Size: 132 238 275
Fragments arranged by size:
275 238 132
BsiHKAI G_wGCw'C
Cuts at: 0 577 645
Size: 577 68
Bsp1286I G_dGCh'C
Cuts at: 0 50 452 577 645
Size: 50 402 125 68
Fragments arranged by size:
402 125 68 50
BspHI T'CATG_A
Cuts at: 0 386 645
Size: 386 259
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 111 423 645
Size: 111 312 222
Fragments arranged by size:
312 222 111
BsrBI CCG'CTC
Cuts at: 0 279 459 645
Size: 279 180 186
Fragments arranged by size:
279 186 180
BstAPI GCAn_nnn'nTGC
Cuts at: 0 221 645
Size: 221 424
BstYI r'GATC_y
Cuts at: 0 422 645
Size: 422 223
BtgI C'CryG_G
Cuts at: 0 408 414 467 645
Size: 408 6 53 178
Fragments arranged by size:
408 178 53 6
EaeI y'GGCC_r
Cuts at: 0 411 473 645
Size: 411 62 172
Fragments arranged by size:
411 172 62
EarI CTCTTCn'nnn_
Cuts at: 0 440 645
Size: 440 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 18 226 289 295 607 645
Size: 18 208 63 6 312 38
Fragments arranged by size:
312 208 63 38 18 6
EcoO109I rG'GnC_Cy
Cuts at: 0 435 645
Size: 435 210
HaeII r_GCGC'y
Cuts at: 0 641 645
Size: 641 4
KasI G'GCGC_C
Cuts at: 0 637 645
Size: 637 8
MscI TGG'CCA
Cuts at: 0 413 645
Size: 413 232
MslI CAynn'nnrTG
Cuts at: 0 8 523 645
Size: 8 515 122
Fragments arranged by size:
515 122 8
NarI GG'CG_CC
Cuts at: 0 638 645
Size: 638 7
NcoI C'CATG_G
Cuts at: 0 414 645
Size: 414 231
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 645
Size: 220 32 393
Fragments arranged by size:
393 220 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 645
Size: 435 210
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 187 219 645
Size: 187 32 426
Fragments arranged by size:
426 187 32
PstI C_TGCA'G
Cuts at: 0 434 645
Size: 434 211
SapI GCTCTTCn'nnn_
Cuts at: 0 440 645
Size: 440 205
SfcI C'TryA_G
Cuts at: 0 430 645
Size: 430 215
SfoI GGC'GCC
Cuts at: 0 639 645
Size: 639 6
StyI C'CwwG_G
Cuts at: 0 414 645
Size: 414 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 475 607 645
Size: 475 132 38
Fragments arranged by size:
475 132 38
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 412 645
Size: 412 233
Enzymes that do cut:
AcuI AlfI AlwNI BanI BanII BbeI BbsI BglI
BlpI BplI BpmI Bpu10I BsaHI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BstAPI BstYI BtgI
EaeI EarI Eco57MI EcoO109I HaeII KasI MscI MslI
NarI NcoI PpiI PpuMI PsrI PstI SapI SfcI
SfoI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI