Restriction map for
pFN21AE5208
(Linear) (Six-base) MAPSORT of: KIEE5208.seq Check: 2072 from: 1 to: 732
KIEE5208.orf
With 182 enzymes: SgfI *
January 29, 2014 11:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 732
Size: 394 338
AflIII A'CryG_T
Cuts at: 0 299 732
Size: 299 433
AlwNI CAG_nnn'CTG
Cuts at: 0 444 491 732
Size: 444 47 241
Fragments arranged by size:
444 241 47
AvaI C'yCGr_G
Cuts at: 0 305 732
Size: 305 427
BanI G'GyrC_C
Cuts at: 0 21 274 529 661 732
Size: 21 253 255 132 71
Fragments arranged by size:
255 253 132 71 21
BanII G_rGCy'C
Cuts at: 0 226 417 732
Size: 226 191 315
Fragments arranged by size:
315 226 191
BbeI G_GCGC'C
Cuts at: 0 278 665 732
Size: 278 387 67
Fragments arranged by size:
387 278 67
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 369 403 732
Size: 369 34 329
Fragments arranged by size:
369 329 34
BlpI GC'TnA_GC
Cuts at: 0 416 732
Size: 416 316
Bme1580I G_kGCm'C
Cuts at: 0 532 732
Size: 532 200
BmgBI CAC'GTC
Cuts at: 0 258 300 732
Size: 258 42 432
Fragments arranged by size:
432 258 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 610 732
Size: 610 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 181 732
Size: 142 39 551
Fragments arranged by size:
551 142 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 553 732
Size: 553 179
BsaHI Gr'CG_yC
Cuts at: 0 275 662 732
Size: 275 387 70
Fragments arranged by size:
387 275 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 418 421 466 721 732
Size: 418 3 45 255 11
Fragments arranged by size:
418 255 45 11 3
BseYI C'CCAG_C
Cuts at: 0 437 732
Size: 437 295
BsiEI CG_ry'CG
Cuts at: 0 393 732
Size: 393 339
BsmI GAATG_Cn'
Cuts at: 0 649 732
Size: 649 83
Bsp1286I G_dGCh'C
Cuts at: 0 226 417 532 732
Size: 226 191 115 200
Fragments arranged by size:
226 200 191 115
BsrBI CCG'CTC
Cuts at: 0 68 365 411 732
Size: 68 297 46 321
Fragments arranged by size:
321 297 68 46
BsrFI r'CCGG_y
Cuts at: 0 392 732
Size: 392 340
BstBI TT'CG_AA
Cuts at: 0 641 732
Size: 641 91
BstEII G'GTnAC_C
Cuts at: 0 30 732
Size: 30 702
BstXI CCAn_nnnn'nTGG
Cuts at: 0 480 732
Size: 480 252
BtgI C'CryG_G
Cuts at: 0 26 479 732
Size: 26 453 253
Fragments arranged by size:
453 253 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 84 732
Size: 84 648
BtsI GCAGTG_nn'
Cuts at: 0 624 732
Size: 624 108
DrdI GACnn_nn'nnGTC
Cuts at: 0 15 732
Size: 15 717
EaeI y'GGCC_r
Cuts at: 0 390 446 732
Size: 390 56 286
Fragments arranged by size:
390 286 56
EagI C'GGCC_G
Cuts at: 0 390 732
Size: 390 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 181 394 732
Size: 142 39 213 338
Fragments arranged by size:
338 213 142 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 448 732
Size: 448 284
HaeII r_GCGC'y
Cuts at: 0 278 444 665 732
Size: 278 166 221 67
Fragments arranged by size:
278 221 166 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 286 318 732
Size: 286 32 414
Fragments arranged by size:
414 286 32
HincII GTy'rAC
Cuts at: 0 177 732
Size: 177 555
KasI G'GCGC_C
Cuts at: 0 274 661 732
Size: 274 387 71
Fragments arranged by size:
387 274 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 732
Size: 701 31
MslI CAynn'nnrTG
Cuts at: 0 478 535 732
Size: 478 57 197
Fragments arranged by size:
478 197 57
MspA1I CmG'CkG
Cuts at: 0 491 732
Size: 491 241
NarI GG'CG_CC
Cuts at: 0 275 662 732
Size: 275 387 70
Fragments arranged by size:
387 275 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 732
Size: 444 288
PfoI T'CCnGG_A
Cuts at: 0 701 732
Size: 701 31
RsrII CG'GwC_CG
Cuts at: 0 360 732
Size: 360 372
SfcI C'TryA_G
Cuts at: 0 189 233 732
Size: 189 44 499
Fragments arranged by size:
499 189 44
SfoI GGC'GCC
Cuts at: 0 276 663 732
Size: 276 387 69
Fragments arranged by size:
387 276 69
SmaI CCC'GGG
Cuts at: 0 307 732
Size: 307 425
StyI C'CwwG_G
Cuts at: 0 282 732
Size: 282 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 732
Size: 39 693
TatI w'GTAC_w
Cuts at: 0 317 732
Size: 317 415
Tth111I GACn'n_nGTC
Cuts at: 0 358 732
Size: 358 374
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 655 732
Size: 655 77
XmaI C'CCGG_G
Cuts at: 0 305 732
Size: 305 427
Enzymes that do cut:
AcuI AflIII AlwNI AvaI BanI BanII BbeI BcgI
BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI BseRI
BseYI BsiEI BsmI Bsp1286I BsrBI BsrFI BstBI BstEII
BstXI BtgI BtgZI BtsI DrdI EaeI EagI Eco57MI
EcoNI HaeII Hin4I HincII KasI MmeI MslI MspA1I
NarI PflMI PfoI RsrII SfcI SfoI SmaI StyI
TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EarI
EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XhoI XmnI ZraI