Restriction map for
pFN21AE5204
(Linear) (Six-base) MAPSORT of: KIEE5204.seq Check: 469 from: 1 to: 624
KIEE5204.orf
With 182 enzymes: SgfI *
January 29, 2014 11:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 557 624
Size: 374 183 67
Fragments arranged by size:
374 183 67
AlwNI CAG_nnn'CTG
Cuts at: 0 225 397 624
Size: 225 172 227
Fragments arranged by size:
227 225 172
ApaI G_GGCC'C
Cuts at: 0 415 624
Size: 415 209
AscI GG'CGCG_CC
Cuts at: 0 254 624
Size: 254 370
AvaI C'yCGr_G
Cuts at: 0 78 357 373 529 583 624
Size: 78 279 16 156 54 41
Fragments arranged by size:
279 156 78 54 41 16
BanI G'GyrC_C
Cuts at: 0 348 624
Size: 348 276
BanII G_rGCy'C
Cuts at: 0 415 424 584 590 624
Size: 415 9 160 6 34
Fragments arranged by size:
415 160 34 9 6
BbvCI CC'TCA_GC
Cuts at: 0 476 624
Size: 476 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 338 372 624
Size: 338 34 252
Fragments arranged by size:
338 252 34
BglI GCCn_nnn'nGGC
Cuts at: 0 419 624
Size: 419 205
Bme1580I G_kGCm'C
Cuts at: 0 240 415 624
Size: 240 175 209
Fragments arranged by size:
240 209 175
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 624
Size: 580 44
Bpu10I CC'TnA_GC
Cuts at: 0 476 624
Size: 476 148
BsaAI yAC'GTr
Cuts at: 0 285 624
Size: 285 339
BsaHI Gr'CG_yC
Cuts at: 0 340 624
Size: 340 284
BsaWI w'CCGG_w
Cuts at: 0 498 570 624
Size: 498 72 54
Fragments arranged by size:
498 72 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 624
Size: 7 30 587
Fragments arranged by size:
587 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 624
Size: 466 158
BseYI C'CCAG_C
Cuts at: 0 239 624
Size: 239 385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 624
Size: 214 410
BsiEI CG_ry'CG
Cuts at: 0 22 39 624
Size: 22 17 585
Fragments arranged by size:
585 22 17
BsiHKAI G_wGCw'C
Cuts at: 0 119 624
Size: 119 505
BsmBI CGTCTCn'nnnn_
Cuts at: 0 55 624
Size: 55 569
Bsp1286I G_dGCh'C
Cuts at: 0 119 240 415 424 584 590 624
Size: 119 121 175 9 160 6 34
Fragments arranged by size:
175 160 121 119 34 9 6
BsrBI CCG'CTC
Cuts at: 0 18 45 624
Size: 18 27 579
Fragments arranged by size:
579 27 18
BssHII G'CGCG_C
Cuts at: 0 254 624
Size: 254 370
BstAPI GCAn_nnn'nTGC
Cuts at: 0 145 624
Size: 145 479
BstXI CCAn_nnnn'nTGG
Cuts at: 0 335 624
Size: 335 289
Bsu36I CC'TnA_GG
Cuts at: 0 416 624
Size: 416 208
BtgI C'CryG_G
Cuts at: 0 248 624
Size: 248 376
BtsI GCAGTG_nn'
Cuts at: 0 148 624
Size: 148 476
EaeI y'GGCC_r
Cuts at: 0 19 624
Size: 19 605
EagI C'GGCC_G
Cuts at: 0 19 624
Size: 19 605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 624
Size: 88 536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 557 580 624
Size: 374 183 23 44
Fragments arranged by size:
374 183 44 23
EcoO109I rG'GnC_Cy
Cuts at: 0 411 412 443 624
Size: 411 1 31 181
Fragments arranged by size:
411 181 31 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 624
Size: 7 32 585
Fragments arranged by size:
585 32 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 624
Size: 71 553
MspA1I CmG'CkG
Cuts at: 0 250 624
Size: 250 374
NspI r_CATG'y
Cuts at: 0 371 624
Size: 371 253
PfoI T'CCnGG_A
Cuts at: 0 439 624
Size: 439 185
PmlI CAC'GTG
Cuts at: 0 285 624
Size: 285 339
PpuMI rG'GwC_Cy
Cuts at: 0 443 624
Size: 443 181
PspOMI G'GGCC_C
Cuts at: 0 411 624
Size: 411 213
PstI C_TGCA'G
Cuts at: 0 71 624
Size: 71 553
SacII CC_GC'GG
Cuts at: 0 251 624
Size: 251 373
SanDI GG'GwC_CC
Cuts at: 0 443 624
Size: 443 181
SfcI C'TryA_G
Cuts at: 0 67 624
Size: 67 557
SmaI CCC'GGG
Cuts at: 0 375 624
Size: 375 249
StuI AGG'CCT
Cuts at: 0 481 624
Size: 481 143
XmaI C'CCGG_G
Cuts at: 0 373 624
Size: 373 251
Enzymes that do cut:
AcuI AlwNI ApaI AscI AvaI BanI BanII BbvCI
BcgI BglI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BsrBI BssHII BstAPI BstXI Bsu36I BtgI BtsI EaeI
EagI EciI Eco57MI EcoO109I Hin4I MmeI MspA1I NspI
PfoI PmlI PpuMI PspOMI PstI SacII SanDI SfcI
SmaI StuI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaBI BsiWI BsmI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI