(Linear) (Six-base) MAPSORT of: KIEE5203.seq  Check: 4001  from: 1  to: 660



KIEE5203.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     124     660 
   Size:         47      77     536

  Fragments arranged by size: 

                 536      77      47
 
ApoI r'AATT_y

Cuts at:      0     595     660 
   Size:        595      65
 
BanII G_rGCy'C

Cuts at:      0     130     350     660 
   Size:        130     220     310

  Fragments arranged by size: 

                 310     220     130
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     518     660 
   Size:        518     142
 
BfrBI ATG'CAT

Cuts at:      0      22     660 
   Size:         22     638
 
BglII A'GATC_T

Cuts at:      0     399     660 
   Size:        399     261
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     660 
   Size:        193     467
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     660 
   Size:        334     326
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     660 
   Size:        360     300
 
BsiHKAI G_wGCw'C

Cuts at:      0      72     660 
   Size:         72     588
 
Bsp1286I G_dGCh'C

Cuts at:      0      72     130     350     660 
   Size:         72      58     220     310

  Fragments arranged by size: 

                 310     220      72      58
 
BstYI r'GATC_y

Cuts at:      0     399     660 
   Size:        399     261
 
BtsI GCAGTG_nn'

Cuts at:      0     348     660 
   Size:        348     312
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      52     660 
   Size:         52     608
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     124     193     660 
   Size:         47      77      69     467

  Fragments arranged by size: 

                 467      77      69      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      90     472     660 
   Size:         90     382     188

  Fragments arranged by size: 

                 382     188      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0      95     333     660 
   Size:         95     238     327

  Fragments arranged by size: 

                 327     238      95
 
MfeI C'AATT_G

Cuts at:      0     650     660 
   Size:        650      10
 
MspA1I CmG'CkG

Cuts at:      0      75     150     660 
   Size:         75      75     510

  Fragments arranged by size: 

                 510      75      75
 
NsiI A_TGCA'T

Cuts at:      0      24     660 
   Size:         24     636
 
PfoI T'CCnGG_A

Cuts at:      0     170     660 
   Size:        170     490
 
PpuMI rG'GwC_Cy

Cuts at:      0     333     660 
   Size:        333     327
 
PstI C_TGCA'G

Cuts at:      0     155     660 
   Size:        155     505
 
PvuII CAG'CTG

Cuts at:      0     150     660 
   Size:        150     510
 
ScaI AGT'ACT

Cuts at:      0     178     660 
   Size:        178     482
 
SfcI C'TryA_G

Cuts at:      0     151     472     660 
   Size:        151     321     188

  Fragments arranged by size: 

                 321     188     151
 
SmlI C'TyrA_G

Cuts at:      0     349     660 
   Size:        349     311
 
StuI AGG'CCT

Cuts at:      0      85     224     660 
   Size:         85     139     436

  Fragments arranged by size: 

                 436     139      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     596     660 
   Size:        596      64
 
TatI w'GTAC_w

Cuts at:      0     176     323     660 
   Size:        176     147     337

  Fragments arranged by size: 

                 337     176     147
 
XmnI GAAnn'nnTTC

Cuts at:      0     631     660 
   Size:        631      29
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BbsI    BfrBI    BglII     BpmI    BpuEI
     BsgI  BsiHKAI Bsp1286I    BstYI     BtsI     DrdI  Eco57MI    EcoNI
 EcoO109I     MfeI   MspA1I     NsiI     PfoI    PpuMI     PstI    PvuII
     ScaI     SfcI     SmlI     StuI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI