(Linear) (Six-base) MAPSORT of: KIEE5189.seq  Check: 1481  from: 1  to: 441



KIEE5189.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     152     441 
   Size:        152     289
 
Bme1580I G_kGCm'C

Cuts at:      0     236     287     441 
   Size:        236      51     154

  Fragments arranged by size: 

                 236     154      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     441 
   Size:        295     146
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     160     441 
   Size:        160     281
 
BsaBI GATnn'nnATC

Cuts at:      0     137     441 
   Size:        137     304
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110     307     441 
   Size:        110     197     134

  Fragments arranged by size: 

                 197     134     110
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     441 
   Size:         62     379
 
BsmI GAATG_Cn'

Cuts at:      0     391     441 
   Size:        391      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     287     441 
   Size:        236      51     154

  Fragments arranged by size: 

                 236     154      51
 
BsrDI GCAATG_nn'

Cuts at:      0     267     441 
   Size:        267     174
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     152     318     441 
   Size:        152     166     123

  Fragments arranged by size: 

                 166     152     123
 
BtgI C'CryG_G

Cuts at:      0      83     441 
   Size:         83     358
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     258     441 
   Size:        258     183
 
DraIII CAC_nnn'GTG

Cuts at:      0     117     441 
   Size:        117     324
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     441 
   Size:        295     146
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     335     441 
   Size:        335     106
 
EcoO109I rG'GnC_Cy

Cuts at:      0     270     441 
   Size:        270     171
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     315     347     441 
   Size:        315      32      94

  Fragments arranged by size: 

                 315      94      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     323     441 
   Size:        323     118
 
MslI CAynn'nnrTG

Cuts at:      0     307     441 
   Size:        307     134
 
NcoI C'CATG_G

Cuts at:      0      83     441 
   Size:         83     358
 
PfoI T'CCnGG_A

Cuts at:      0     185     441 
   Size:        185     256
 
SfcI C'TryA_G

Cuts at:      0     367     441 
   Size:        367      74
 
StyI C'CwwG_G

Cuts at:      0      83     336     441 
   Size:         83     253     105

  Fragments arranged by size: 

                 253     105      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       9      96     441 
   Size:          9      87     345

  Fragments arranged by size: 

                 345      87       9
 
TatI w'GTAC_w

Cuts at:      0     209     441 
   Size:        209     232
 

 Enzymes that do cut:

    AlwNI Bme1580I     BpmI     BsaI    BsaBI    BseRI     BsgI     BsmI
 Bsp1286I    BsrDI   BstAPI     BtgI    BtgZI   DraIII  Eco57MI    EcoNI
 EcoO109I    Hin4I     MmeI     MslI     NcoI     PfoI     SfcI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI