(Linear) (Six-base) MAPSORT of: KIEE5188.seq  Check: 8641  from: 1  to: 387



KIEE5188.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AccI GT'mk_AC

Cuts at:      0     368     387 
   Size:        368      19
 
ApoI r'AATT_y

Cuts at:      0     230     350     387 
   Size:        230     120      37

  Fragments arranged by size: 

                 230     120      37
 
BclI T'GATC_A

Cuts at:      0      66     387 
   Size:         66     321
 
BfrBI ATG'CAT

Cuts at:      0      45     387 
   Size:         45     342
 
BglI GCCn_nnn'nGGC

Cuts at:      0     100     387 
   Size:        100     287
 
BlpI GC'TnA_GC

Cuts at:      0     178     387 
   Size:        178     209
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     387 
   Size:        247     140
 
BsaAI yAC'GTr

Cuts at:      0       6     387 
   Size:          6     381
 
BstYI r'GATC_y

Cuts at:      0      21     387 
   Size:         21     366
 
EarI CTCTTCn'nnn_

Cuts at:      0     218     387 
   Size:        218     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     387 
   Size:        247     140
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     387 
   Size:          6      32     349

  Fragments arranged by size: 

                 349      32       6
 
HincII GTy'rAC

Cuts at:      0      87     387 
   Size:         87     300
 
HindIII A'AGCT_T

Cuts at:      0     156     387 
   Size:        156     231
 
HpaI GTT'AAC

Cuts at:      0      87     387 
   Size:         87     300
 
MspA1I CmG'CkG

Cuts at:      0     177     387 
   Size:        177     210
 
NsiI A_TGCA'T

Cuts at:      0      47     387 
   Size:         47     340
 
PfoI T'CCnGG_A

Cuts at:      0     263     387 
   Size:        263     124
 
PvuII CAG'CTG

Cuts at:      0     177     387 
   Size:        177     210
 
XbaI T'CTAG_A

Cuts at:      0     219     387 
   Size:        219     168
 

 Enzymes that do cut:

     AccI     ApoI     BclI    BfrBI     BglI     BlpI     BpmI    BsaAI
    BstYI     EarI  Eco57MI    Hin4I   HincII  HindIII     HpaI   MspA1I
     NsiI     PfoI    PvuII     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI