(Linear) (Six-base) MAPSORT of: KIEE5185.seq  Check: 6920  from: 1  to: 390



KIEE5185.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:50  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     365     390 
   Size:        148     217      25

  Fragments arranged by size: 

                 217     148      25
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      89     390 
   Size:         89     301
 
ApaLI G'TGCA_C

Cuts at:      0     171     390 
   Size:        171     219
 
BamHI G'GATC_C

Cuts at:      0     105     390 
   Size:        105     285
 
BlpI GC'TnA_GC

Cuts at:      0     349     390 
   Size:        349      41
 
Bme1580I G_kGCm'C

Cuts at:      0     175     390 
   Size:        175     215
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     390 
   Size:        337      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     390 
   Size:        310      30      50

  Fragments arranged by size: 

                 310      50      30
 
BseYI C'CCAG_C

Cuts at:      0     344     390 
   Size:        344      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     175     390 
   Size:        175     215
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     390 
   Size:        175     215
 
BssSI C'ACGA_G

Cuts at:      0     215     390 
   Size:        215     175
 
BstYI r'GATC_y

Cuts at:      0     105     276     390 
   Size:        105     171     114

  Fragments arranged by size: 

                 171     114     105
 
BtsI GCAGTG_nn'

Cuts at:      0     175     390 
   Size:        175     215
 
EarI CTCTTCn'nnn_

Cuts at:      0     384     390 
   Size:        384       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     337     365     390 
   Size:        148     189      28      25

  Fragments arranged by size: 

                 189     148      28      25
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     390 
   Size:        243     147
 
NspI r_CATG'y

Cuts at:      0     229     390 
   Size:        229     161
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     358     390 
   Size:        358      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36      99     390 
   Size:         36      63     291

  Fragments arranged by size: 

                 291      63      36
 

 Enzymes that do cut:

     AcuI     AlfI    ApaLI    BamHI     BlpI Bme1580I     BpmI    BsaXI
    BseYI  BsiHKAI Bsp1286I    BssSI    BstYI     BtsI     EarI  Eco57MI
     MmeI     NspI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI