(Linear) (Six-base) MAPSORT of: KIEE5183.seq  Check: 8131  from: 1  to: 345



KIEE5183.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AatII G_ACGT'C

Cuts at:      0     161     345 
   Size:        161     184
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     345 
   Size:        326      19
 
AlwNI CAG_nnn'CTG

Cuts at:      0     235     244     345 
   Size:        235       9     101

  Fragments arranged by size: 

                 235     101       9
 
AvrII C'CTAG_G

Cuts at:      0      33     345 
   Size:         33     312
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     161     345 
   Size:        161     184
 
BglII A'GATC_T

Cuts at:      0      74     345 
   Size:         74     271
 
BsaHI Gr'CG_yC

Cuts at:      0     158     345 
   Size:        158     187
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28     345 
   Size:         28     317
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      36     289     313     345 
   Size:         36     253      24      32

  Fragments arranged by size: 

                 253      36      32      24
 
BseYI C'CCAG_C

Cuts at:      0      52     345 
   Size:         52     293
 
BsiEI CG_ry'CG

Cuts at:      0     111     345 
   Size:        111     234
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     222     345 
   Size:        222     123
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      29     204     345 
   Size:         29     175     141

  Fragments arranged by size: 

                 175     141      29
 
BsrDI GCAATG_nn'

Cuts at:      0     161     345 
   Size:        161     184
 
BsrFI r'CCGG_y

Cuts at:      0     110     345 
   Size:        110     235
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     244     345 
   Size:        244     101
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     175     345 
   Size:        175     170
 
BstYI r'GATC_y

Cuts at:      0      74     345 
   Size:         74     271
 
EarI CTCTTCn'nnn_

Cuts at:      0     302     345 
   Size:        302      43
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     115     345 
   Size:        115     230
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     345 
   Size:        326      19
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      93     345 
   Size:         93     252
 
HaeII r_GCGC'y

Cuts at:      0      59     345 
   Size:         59     286
 
HindIII A'AGCT_T

Cuts at:      0     181     345 
   Size:        181     164
 
PasI CC'CwG_GG

Cuts at:      0     168     345 
   Size:        168     177
 
PstI C_TGCA'G

Cuts at:      0     240     270     345 
   Size:        240      30      75

  Fragments arranged by size: 

                 240      75      30
 
SfcI C'TryA_G

Cuts at:      0     209     236     266     345 
   Size:        209      27      30      79

  Fragments arranged by size: 

                 209      79      30      27
 
StyI C'CwwG_G

Cuts at:      0      33      59     345 
   Size:         33      26     286

  Fragments arranged by size: 

                 286      33      26
 
ZraI GAC'GTC

Cuts at:      0     159     345 
   Size:        159     186
 

 Enzymes that do cut:

    AatII     AcuI    AlwNI    AvrII     BbsI    BglII    BsaHI    BsaXI
    BseRI    BseYI    BsiEI    BsmBI    BspMI    BsrDI    BsrFI   BstAPI
    BstXI    BstYI     EarI     EciI  Eco57MI     FalI    HaeII  HindIII
     PasI     PstI     SfcI     StyI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI     BsgI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    Hin4I   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI