Restriction map for
pFN21AE5178
(Linear) (Six-base) MAPSORT of: KIEE5178.seq Check: 6796 from: 1 to: 459
KIEE5178.orf
With 182 enzymes: SgfI *
January 29, 2014 11:49 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 459
Size: 374 85
AfeI AGC'GCT
Cuts at: 0 363 459
Size: 363 96
AvaI C'yCGr_G
Cuts at: 0 106 169 334 388 459
Size: 106 63 165 54 71
Fragments arranged by size:
165 106 71 63 54
BamHI G'GATC_C
Cuts at: 0 128 459
Size: 128 331
BanII G_rGCy'C
Cuts at: 0 352 459
Size: 352 107
BglI GCCn_nnn'nGGC
Cuts at: 0 356 459
Size: 356 103
BglII A'GATC_T
Cuts at: 0 324 459
Size: 324 135
Bme1580I G_kGCm'C
Cuts at: 0 191 459
Size: 191 268
BsaAI yAC'GTr
Cuts at: 0 288 459
Size: 288 171
BsaHI Gr'CG_yC
Cuts at: 0 437 459
Size: 437 22
BsaWI w'CCGG_w
Cuts at: 0 125 459
Size: 125 334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 409 459
Size: 406 3 50
Fragments arranged by size:
406 50 3
BseYI C'CCAG_C
Cuts at: 0 33 101 442 459
Size: 33 68 341 17
Fragments arranged by size:
341 68 33 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 235 310 459
Size: 139 96 75 149
Fragments arranged by size:
149 139 96 75
Bsp1286I G_dGCh'C
Cuts at: 0 191 352 459
Size: 191 161 107
Fragments arranged by size:
191 161 107
BspEI T'CCGG_A
Cuts at: 0 125 459
Size: 125 334
BsrFI r'CCGG_y
Cuts at: 0 29 459
Size: 29 430
BstYI r'GATC_y
Cuts at: 0 128 324 459
Size: 128 196 135
Fragments arranged by size:
196 135 128
Bsu36I CC'TnA_GG
Cuts at: 0 157 459
Size: 157 302
BtgI C'CryG_G
Cuts at: 0 134 191 300 424 459
Size: 134 57 109 124 35
Fragments arranged by size:
134 124 109 57 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 459
Size: 277 182
EarI CTCTTCn'nnn_
Cuts at: 0 231 459
Size: 231 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 459
Size: 374 85
HaeII r_GCGC'y
Cuts at: 0 365 459
Size: 365 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 459
Size: 225 32 202
Fragments arranged by size:
225 202 32
MslI CAynn'nnrTG
Cuts at: 0 139 459
Size: 139 320
MspA1I CmG'CkG
Cuts at: 0 81 294 426 446 459
Size: 81 213 132 20 13
Fragments arranged by size:
213 132 81 20 13
NcoI C'CATG_G
Cuts at: 0 134 300 459
Size: 134 166 159
Fragments arranged by size:
166 159 134
NspI r_CATG'y
Cuts at: 0 387 459
Size: 387 72
PspXI vC'TCGA_Gb
Cuts at: 0 169 388 459
Size: 169 219 71
Fragments arranged by size:
219 169 71
PvuII CAG'CTG
Cuts at: 0 81 294 446 459
Size: 81 213 152 13
Fragments arranged by size:
213 152 81 13
SacII CC_GC'GG
Cuts at: 0 427 459
Size: 427 32
SfcI C'TryA_G
Cuts at: 0 378 459
Size: 378 81
SmlI C'TyrA_G
Cuts at: 0 169 388 459
Size: 169 219 71
Fragments arranged by size:
219 169 71
SphI G_CATG'C
Cuts at: 0 387 459
Size: 387 72
StyI C'CwwG_G
Cuts at: 0 134 300 459
Size: 134 166 159
Fragments arranged by size:
166 159 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 459
Size: 25 434
XhoI C'TCGA_G
Cuts at: 0 169 388 459
Size: 169 219 71
Fragments arranged by size:
219 169 71
Enzymes that do cut:
AcuI AfeI AvaI BamHI BanII BglI BglII Bme1580I
BsaAI BsaHI BsaWI BseRI BseYI BsgI Bsp1286I BspEI
BsrFI BstYI Bsu36I BtgI DrdI EarI Eco57MI HaeII
Hin4I MslI MspA1I NcoI NspI PspXI PvuII SacII
SfcI SmlI SphI StyI TaqII XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI