(Linear) (Six-base) MAPSORT of: KIEE5177.seq  Check: 4212  from: 1  to: 453



KIEE5177.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AleI CACnn'nnGTG

Cuts at:      0      14     453 
   Size:         14     439
 
ApoI r'AATT_y

Cuts at:      0     365     453 
   Size:        365      88
 
BanI G'GyrC_C

Cuts at:      0     339     453 
   Size:        339     114
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     444     453 
   Size:        444       9
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     453 
   Size:         40     413
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     286     453 
   Size:        286     167
 
BsmI GAATG_Cn'

Cuts at:      0     143     452     453 
   Size:        143     309       1

  Fragments arranged by size: 

                 309     143       1
 
BsrDI GCAATG_nn'

Cuts at:      0     277     453 
   Size:        277     176
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     276     346     453 
   Size:        276      70     107

  Fragments arranged by size: 

                 276     107      70
 
BstEII G'GTnAC_C

Cuts at:      0      49     453 
   Size:         49     404
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     453 
   Size:         40     413
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     247     453 
   Size:        247     206
 
EcoO109I rG'GnC_Cy

Cuts at:      0     311     453 
   Size:        311     142
 
MfeI C'AATT_G

Cuts at:      0     386     453 
   Size:        386      67
 
MslI CAynn'nnrTG

Cuts at:      0      14     199     453 
   Size:         14     185     254

  Fragments arranged by size: 

                 254     185      14
 
NspI r_CATG'y

Cuts at:      0     331     453 
   Size:        331     122
 
PfoI T'CCnGG_A

Cuts at:      0     172     453 
   Size:        172     281
 
PpuMI rG'GwC_Cy

Cuts at:      0     311     453 
   Size:        311     142
 
PstI C_TGCA'G

Cuts at:      0     281     453 
   Size:        281     172
 
SfcI C'TryA_G

Cuts at:      0     223     277     453 
   Size:        223      54     176

  Fragments arranged by size: 

                 223     176      54
 
SmlI C'TyrA_G

Cuts at:      0     265     453 
   Size:        265     188
 
SphI G_CATG'C

Cuts at:      0     331     453 
   Size:        331     122
 
Tth111I GACn'n_nGTC

Cuts at:      0     420     453 
   Size:        420      33
 

 Enzymes that do cut:

     AleI     ApoI     BanI     BplI     BpmI    BpuEI     BsmI    BsrDI
   BstAPI   BstEII  Eco57MI    EcoNI EcoO109I     MfeI     MslI     NspI
     PfoI    PpuMI     PstI     SfcI     SmlI     SphI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI