(Linear) (Six-base) MAPSORT of: KIEE5175.seq  Check: 1507  from: 1  to: 408



KIEE5175.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     357     408 
   Size:        357      51
 
AatII G_ACGT'C

Cuts at:      0      67     408 
   Size:         67     341
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     408 
   Size:        301     107
 
AleI CACnn'nnGTG

Cuts at:      0     208     408 
   Size:        208     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     349     408 
   Size:         27     322      59

  Fragments arranged by size: 

                 322      59      27
 
BanII G_rGCy'C

Cuts at:      0     152     217     408 
   Size:        152      65     191

  Fragments arranged by size: 

                 191     152      65
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     319     408 
   Size:        319      89
 
BsaHI Gr'CG_yC

Cuts at:      0      64     408 
   Size:         64     344
 
BsiEI CG_ry'CG

Cuts at:      0     105     408 
   Size:        105     303
 
BsiHKAI G_wGCw'C

Cuts at:      0     217     408 
   Size:        217     191
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     217     408 
   Size:        152      65     191

  Fragments arranged by size: 

                 191     152      65
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     357     408 
   Size:        357      51
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     349     408 
   Size:        349      59
 
EarI CTCTTCn'nnn_

Cuts at:      0     280     408 
   Size:        280     128
 
EcoICRI GAG'CTC

Cuts at:      0     215     408 
   Size:        215     193
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     408 
   Size:        301     107
 
MslI CAynn'nnrTG

Cuts at:      0     208     408 
   Size:        208     200
 
SacI G_AGCT'C

Cuts at:      0     217     408 
   Size:        217     191
 
StyI C'CwwG_G

Cuts at:      0     218     408 
   Size:        218     190
 
ZraI GAC'GTC

Cuts at:      0      65     408 
   Size:         65     343
 

 Enzymes that do cut:

     AarI    AatII     AcuI     AleI    AlwNI    BanII     BbsI    BsaHI
    BsiEI  BsiHKAI Bsp1286I    BspMI   BstAPI     EarI  EcoICRI  Eco57MI
     MslI     SacI     StyI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI