Restriction map for
pFN21AE5174
(Linear) (Six-base) MAPSORT of: KIEE5174.seq Check: 3859 from: 1 to: 498
KIEE5174.orf
With 182 enzymes: SgfI *
January 29, 2014 11:49 ..
AccI GT'mk_AC
Cuts at: 0 287 498
Size: 287 211
Acc65I G'GTAC_C
Cuts at: 0 433 498
Size: 433 65
AgeI A'CCGG_T
Cuts at: 0 43 498
Size: 43 455
ApaI G_GGCC'C
Cuts at: 0 94 357 498
Size: 94 263 141
Fragments arranged by size:
263 141 94
BamHI G'GATC_C
Cuts at: 0 167 498
Size: 167 331
BanI G'GyrC_C
Cuts at: 0 433 498
Size: 433 65
BanII G_rGCy'C
Cuts at: 0 94 357 388 498
Size: 94 263 31 110
Fragments arranged by size:
263 110 94 31
BbsI GAAGACnn'nnnn_
Cuts at: 0 406 498
Size: 406 92
Bme1580I G_kGCm'C
Cuts at: 0 94 357 498
Size: 94 263 141
Fragments arranged by size:
263 141 94
BmgBI CAC'GTC
Cuts at: 0 369 498
Size: 369 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 498
Size: 80 418
BsaI GGTCTCn'nnnn_
Cuts at: 0 221 498
Size: 221 277
BsaBI GATnn'nnATC
Cuts at: 0 131 498
Size: 131 367
BsaWI w'CCGG_w
Cuts at: 0 43 329 498
Size: 43 286 169
Fragments arranged by size:
286 169 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 30 60 136 166 498
Size: 30 30 76 30 332
Fragments arranged by size:
332 76 30 30 30
BseYI C'CCAG_C
Cuts at: 0 393 498
Size: 393 105
Bsp1286I G_dGCh'C
Cuts at: 0 94 357 388 498
Size: 94 263 31 110
Fragments arranged by size:
263 110 94 31
BspEI T'CCGG_A
Cuts at: 0 329 498
Size: 329 169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 251 498
Size: 251 247
BsrFI r'CCGG_y
Cuts at: 0 43 156 311 498
Size: 43 113 155 187
Fragments arranged by size:
187 155 113 43
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 498
Size: 114 384
BstYI r'GATC_y
Cuts at: 0 167 174 498
Size: 167 7 324
Fragments arranged by size:
324 167 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 498
Size: 80 418
EcoO109I rG'GnC_Cy
Cuts at: 0 90 91 354 498
Size: 90 1 263 144
Fragments arranged by size:
263 144 90 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 498
Size: 471 27
HaeII r_GCGC'y
Cuts at: 0 150 498
Size: 150 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 498
Size: 159 32 307
Fragments arranged by size:
307 159 32
KpnI G_GTAC'C
Cuts at: 0 437 498
Size: 437 61
MspA1I CmG'CkG
Cuts at: 0 54 390 498
Size: 54 336 108
Fragments arranged by size:
336 108 54
PasI CC'CwG_GG
Cuts at: 0 87 498
Size: 87 411
PshAI GACnn'nnGTC
Cuts at: 0 406 498
Size: 406 92
PspOMI G'GGCC_C
Cuts at: 0 90 353 498
Size: 90 263 145
Fragments arranged by size:
263 145 90
PstI C_TGCA'G
Cuts at: 0 362 498
Size: 362 136
SfcI C'TryA_G
Cuts at: 0 358 498
Size: 358 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 252 427 498
Size: 252 175 71
Fragments arranged by size:
252 175 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 30 62 498
Size: 30 32 436
Fragments arranged by size:
436 32 30
XmnI GAAnn'nnTTC
Cuts at: 0 377 493 498
Size: 377 116 5
Fragments arranged by size:
377 116 5
Enzymes that do cut:
AccI Acc65I AgeI ApaI BamHI BanI BanII BbsI
Bme1580I BmgBI BpmI BsaI BsaBI BsaWI BsaXI BseYI
Bsp1286I BspEI BspMI BsrFI BstXI BstYI Eco57MI EcoO109I
FalI HaeII Hin4I KpnI MspA1I PasI PshAI PspOMI
PstI SfcI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BplI
Bpu10I BpuEI BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI