(Linear) (Six-base) MAPSORT of: KIEE5172.seq  Check: 5226  from: 1  to: 486



KIEE5172.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AclI AA'CG_TT

Cuts at:      0     414     486 
   Size:        414      72
 
AflIII A'CryG_T

Cuts at:      0     425     486 
   Size:        425      61
 
AlwNI CAG_nnn'CTG

Cuts at:      0      51     486 
   Size:         51     435
 
AvaI C'yCGr_G

Cuts at:      0      85     486 
   Size:         85     401
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     412     486 
   Size:        412      74
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      66     100     486 
   Size:         66      34     386

  Fragments arranged by size: 

                 386      66      34
 
BclI T'GATC_A

Cuts at:      0      32     486 
   Size:         32     454
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     486 
   Size:        385     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       1     486 
   Size:          1     485
 
BsaHI Gr'CG_yC

Cuts at:      0      59     486 
   Size:         59     427
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     326     486 
   Size:        326     160
 
BseYI C'CCAG_C

Cuts at:      0      73     486 
   Size:         73     413
 
BsiEI CG_ry'CG

Cuts at:      0     126     486 
   Size:        126     360
 
BsrBI CCG'CTC

Cuts at:      0     273     486 
   Size:        273     213
 
BtgI C'CryG_G

Cuts at:      0      11     486 
   Size:         11     475
 
ClaI AT'CG_AT

Cuts at:      0      92     486 
   Size:         92     394
 
EaeI y'GGCC_r

Cuts at:      0     123     486 
   Size:        123     363
 
EagI C'GGCC_G

Cuts at:      0     123     486 
   Size:        123     363
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19     486 
   Size:         19     467
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     486 
   Size:        385     101
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     308     486 
   Size:        308     178
 
NspI r_CATG'y

Cuts at:      0     429     484     486 
   Size:        429      55       2

  Fragments arranged by size: 

                 429      55       2
 
PasI CC'CwG_GG

Cuts at:      0     277     486 
   Size:        277     209
 
PciI A'CATG_T

Cuts at:      0     425     486 
   Size:        425      61
 
PsiI TTA'TAA

Cuts at:      0     376     486 
   Size:        376     110
 
SmlI C'TyrA_G

Cuts at:      0      85     486 
   Size:         85     401
 
SspI AAT'ATT

Cuts at:      0     326     486 
   Size:        326     160
 
XhoI C'TCGA_G

Cuts at:      0      85     486 
   Size:         85     401
 

 Enzymes that do cut:

     AclI   AflIII    AlwNI     AvaI     BbsI     BcgI     BclI     BpmI
     BsaI    BsaHI    BseRI    BseYI    BsiEI    BsrBI     BtgI     ClaI
     EaeI     EagI     EciI  Eco57MI    EcoNI     NspI     PasI     PciI
     PsiI     SmlI     SspI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaWI
    BsaXI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EarI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI