(Linear) (Six-base) MAPSORT of: KIEE5169.seq  Check: 37  from: 1  to: 471



KIEE5169.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AccI GT'mk_AC

Cuts at:      0     369     471 
   Size:        369     102
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     471 
   Size:         32     439
 
ApoI r'AATT_y

Cuts at:      0     157     471 
   Size:        157     314
 
AseI AT'TA_AT

Cuts at:      0     316     471 
   Size:        316     155
 
BamHI G'GATC_C

Cuts at:      0      63     471 
   Size:         63     408
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     385     471 
   Size:        385      86
 
BglII A'GATC_T

Cuts at:      0     456     471 
   Size:        456      15
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     471 
   Size:        358     113
 
Bpu10I CC'TnA_GC

Cuts at:      0     164     471 
   Size:        164     307
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     471 
   Size:        449      22
 
BsaBI GATnn'nnATC

Cuts at:      0     209     471 
   Size:        209     262
 
BsiHKAI G_wGCw'C

Cuts at:      0     447     471 
   Size:        447      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     447     471 
   Size:        447      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     382     471 
   Size:        382      89
 
BstYI r'GATC_y

Cuts at:      0      63     456     471 
   Size:         63     393      15

  Fragments arranged by size: 

                 393      63      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      32     358     471 
   Size:         32     326     113

  Fragments arranged by size: 

                 326     113      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     372     404     471 
   Size:        372      32      67

  Fragments arranged by size: 

                 372      67      32
 
HincII GTy'rAC

Cuts at:      0     370     471 
   Size:        370     101
 
HindIII A'AGCT_T

Cuts at:      0     118     471 
   Size:        118     353
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     471 
   Size:         41     430
 
PfoI T'CCnGG_A

Cuts at:      0      71     471 
   Size:         71     400
 
SalI G'TCGA_C

Cuts at:      0     368     471 
   Size:        368     103
 
SmlI C'TyrA_G

Cuts at:      0     464     471 
   Size:        464       7
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     AseI    BamHI     BbsI    BglII     BpmI
   Bpu10I    BpuEI    BsaBI  BsiHKAI Bsp1286I    BstXI    BstYI  Eco57MI
    Hin4I   HincII  HindIII     MmeI     PfoI     SalI     SmlI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI