(Linear) (Six-base) MAPSORT of: KIEE5168.seq  Check: 2683  from: 1  to: 459



KIEE5168.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AccI GT'mk_AC

Cuts at:      0     269     459 
   Size:        269     190
 
AleI CACnn'nnGTG

Cuts at:      0     373     459 
   Size:        373      86
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78     111     444     459 
   Size:         78      33     333      15

  Fragments arranged by size: 

                 333      78      33      15
 
ApaI G_GGCC'C

Cuts at:      0      32     459 
   Size:         32     427
 
ApoI r'AATT_y

Cuts at:      0     205     303     459 
   Size:        205      98     156

  Fragments arranged by size: 

                 205     156      98
 
AvaI C'yCGr_G

Cuts at:      0      31     459 
   Size:         31     428
 
BanII G_rGCy'C

Cuts at:      0      32     459 
   Size:         32     427
 
BfrBI ATG'CAT

Cuts at:      0     149     459 
   Size:        149     310
 
Bme1580I G_kGCm'C

Cuts at:      0      32     459 
   Size:         32     427
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277     459 
   Size:        277     182
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     459 
   Size:        448      11
 
Bpu10I CC'TnA_GC

Cuts at:      0      38     459 
   Size:         38     421
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     424     454     459 
   Size:        424      30       5

  Fragments arranged by size: 

                 424      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     412     445     459 
   Size:        412      33      14

  Fragments arranged by size: 

                 412      33      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365     457     459 
   Size:        365      92       2

  Fragments arranged by size: 

                 365      92       2
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     459 
   Size:         32     427
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     444     459 
   Size:        444      15
 
BstYI r'GATC_y

Cuts at:      0      18     459 
   Size:         18     441
 
BstZ17I GTA'TAC

Cuts at:      0     270     459 
   Size:        270     189
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      84     459 
   Size:         84     375
 
BtsI GCAGTG_nn'

Cuts at:      0     253     459 
   Size:        253     206
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      58     459 
   Size:         58     401
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     459 
   Size:        448      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     430     459 
   Size:        430      29
 
HaeII r_GCGC'y

Cuts at:      0     366     459 
   Size:        366      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     424     456     459 
   Size:        424      32       3

  Fragments arranged by size: 

                 424      32       3
 
HincII GTy'rAC

Cuts at:      0      87     213     459 
   Size:         87     126     246

  Fragments arranged by size: 

                 246     126      87
 
MslI CAynn'nnrTG

Cuts at:      0     373     459 
   Size:        373      86
 
NsiI A_TGCA'T

Cuts at:      0     151     459 
   Size:        151     308
 
PspOMI G'GGCC_C

Cuts at:      0      28     459 
   Size:         28     431
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     314     331     459 
   Size:        314      17     128

  Fragments arranged by size: 

                 314     128      17
 

 Enzymes that do cut:

     AccI     AleI    AlwNI     ApaI     ApoI     AvaI    BanII    BfrBI
 Bme1580I     BmrI     BpmI   Bpu10I    BsaXI    BseRI     BsgI Bsp1286I
   BstAPI    BstYI  BstZ17I    BtgZI     BtsI     EciI  Eco57MI    EcoNI
    HaeII    Hin4I   HincII     MslI     NsiI   PspOMI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI