(Linear) (Six-base) MAPSORT of: KIEE5167.seq  Check: 3635  from: 1  to: 468



KIEE5167.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     283     468 
   Size:        283     185
 
Acc65I G'GTAC_C

Cuts at:      0     356     468 
   Size:        356     112
 
AleI CACnn'nnGTG

Cuts at:      0     220     468 
   Size:        220     248
 
ApaLI G'TGCA_C

Cuts at:      0     223     468 
   Size:        223     245
 
ApoI r'AATT_y

Cuts at:      0     103     468 
   Size:        103     365
 
AvaI C'yCGr_G

Cuts at:      0      92     468 
   Size:         92     376
 
BanI G'GyrC_C

Cuts at:      0     176     356     468 
   Size:        176     180     112

  Fragments arranged by size: 

                 180     176     112
 
BanII G_rGCy'C

Cuts at:      0     233     424     468 
   Size:        233     191      44

  Fragments arranged by size: 

                 233     191      44
 
BbeI G_GCGC'C

Cuts at:      0     180     468 
   Size:        180     288
 
Bme1580I G_kGCm'C

Cuts at:      0     227     468 
   Size:        227     241
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     297     336     468 
   Size:         28     269      39     132

  Fragments arranged by size: 

                 269     132      39      28
 
BsaHI Gr'CG_yC

Cuts at:      0     177     468 
   Size:        177     291
 
BsaWI w'CCGG_w

Cuts at:      0     374     468 
   Size:        374      94
 
BseYI C'CCAG_C

Cuts at:      0      57      62     139     468 
   Size:         57       5      77     329

  Fragments arranged by size: 

                 329      77      57       5
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     468 
   Size:        227     241
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     233     424     468 
   Size:        227       6     191      44

  Fragments arranged by size: 

                 227     191      44       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     283     468 
   Size:        283     185
 
BssSI C'ACGA_G

Cuts at:      0     226     468 
   Size:        226     242
 
BstEII G'GTnAC_C

Cuts at:      0     270     468 
   Size:        270     198
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     297     336     468 
   Size:         28     269      39     132

  Fragments arranged by size: 

                 269     132      39      28
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      82     468 
   Size:         82     386
 
HaeII r_GCGC'y

Cuts at:      0     180     468 
   Size:        180     288
 
KasI G'GCGC_C

Cuts at:      0     176     468 
   Size:        176     292
 
KpnI G_GTAC'C

Cuts at:      0     360     468 
   Size:        360     108
 
MslI CAynn'nnrTG

Cuts at:      0     220     403     443     468 
   Size:        220     183      40      25

  Fragments arranged by size: 

                 220     183      40      25
 
MspA1I CmG'CkG

Cuts at:      0     111     468 
   Size:        111     357
 
NarI GG'CG_CC

Cuts at:      0     177     468 
   Size:        177     291
 
NdeI CA'TA_TG

Cuts at:      0     119     426     468 
   Size:        119     307      42

  Fragments arranged by size: 

                 307     119      42
 
NspI r_CATG'y

Cuts at:      0     321     468 
   Size:        321     147
 
PasI CC'CwG_GG

Cuts at:      0      78     468 
   Size:         78     390
 
PfoI T'CCnGG_A

Cuts at:      0     260     468 
   Size:        260     208
 
SfoI GGC'GCC

Cuts at:      0     178     468 
   Size:        178     290
 
SmaI CCC'GGG

Cuts at:      0      94     468 
   Size:         94     374
 
SphI G_CATG'C

Cuts at:      0     321     468 
   Size:        321     147
 
XmaI C'CCGG_G

Cuts at:      0      92     468 
   Size:         92     376
 

 Enzymes that do cut:

     AarI   Acc65I     AleI    ApaLI     ApoI     AvaI     BanI    BanII
     BbeI Bme1580I     BpmI    BsaHI    BsaWI    BseYI  BsiHKAI Bsp1286I
    BspMI    BssSI   BstEII  Eco57MI    EcoNI    HaeII     KasI     KpnI
     MslI   MspA1I     NarI     NdeI     NspI     PasI     PfoI     SfoI
     SmaI     SphI     XmaI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     MfeI     MluI     MmeI     MscI     NaeI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI