Restriction map for
pFN21AE5165
(Linear) (Six-base) MAPSORT of: KIEE5165.seq Check: 5537 from: 1 to: 420
KIEE5165.orf
With 182 enzymes: SgfI *
January 29, 2014 11:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 279 420
Size: 279 141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 420
Size: 387 33
AflIII A'CryG_T
Cuts at: 0 191 420
Size: 191 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 311 420
Size: 311 109
AlwNI CAG_nnn'CTG
Cuts at: 0 162 420
Size: 162 258
ApaI G_GGCC'C
Cuts at: 0 180 420
Size: 180 240
AvaI C'yCGr_G
Cuts at: 0 179 420
Size: 179 241
BamHI G'GATC_C
Cuts at: 0 45 368 420
Size: 45 323 52
Fragments arranged by size:
323 52 45
BanII G_rGCy'C
Cuts at: 0 20 180 420
Size: 20 160 240
Fragments arranged by size:
240 160 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 393 420
Size: 130 263 27
Fragments arranged by size:
263 130 27
BglI GCCn_nnn'nGGC
Cuts at: 0 145 420
Size: 145 275
BglII A'GATC_T
Cuts at: 0 203 420
Size: 203 217
Bme1580I G_kGCm'C
Cuts at: 0 180 420
Size: 180 240
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 420
Size: 352 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 90 334 420
Size: 90 244 86
Fragments arranged by size:
244 90 86
BseYI C'CCAG_C
Cuts at: 0 79 140 420
Size: 79 61 280
Fragments arranged by size:
280 79 61
BsiHKAI G_wGCw'C
Cuts at: 0 199 420
Size: 199 221
Bsp1286I G_dGCh'C
Cuts at: 0 20 180 199 420
Size: 20 160 19 221
Fragments arranged by size:
221 160 20 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 279 420
Size: 279 141
BstYI r'GATC_y
Cuts at: 0 45 203 368 420
Size: 45 158 165 52
Fragments arranged by size:
165 158 52 45
Bsu36I CC'TnA_GG
Cuts at: 0 64 420
Size: 64 356
BtgI C'CryG_G
Cuts at: 0 21 311 420
Size: 21 290 109
Fragments arranged by size:
290 109 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 234 420
Size: 234 186
DrdI GACnn_nn'nnGTC
Cuts at: 0 112 420
Size: 112 308
EaeI y'GGCC_r
Cuts at: 0 227 420
Size: 227 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 387 420
Size: 352 35 33
Fragments arranged by size:
352 35 33
EcoO109I rG'GnC_Cy
Cuts at: 0 176 281 420
Size: 176 105 139
Fragments arranged by size:
176 139 105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 420
Size: 147 273
HincII GTy'rAC
Cuts at: 0 117 420
Size: 117 303
MluI A'CGCG_T
Cuts at: 0 191 420
Size: 191 229
MscI TGG'CCA
Cuts at: 0 229 420
Size: 229 191
MspA1I CmG'CkG
Cuts at: 0 23 144 152 261 420
Size: 23 121 8 109 159
Fragments arranged by size:
159 121 109 23 8
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 420
Size: 181 32 207
Fragments arranged by size:
207 181 32
PpuMI rG'GwC_Cy
Cuts at: 0 281 420
Size: 281 139
PspOMI G'GGCC_C
Cuts at: 0 176 420
Size: 176 244
PstI C_TGCA'G
Cuts at: 0 260 266 420
Size: 260 6 154
Fragments arranged by size:
260 154 6
PvuII CAG'CTG
Cuts at: 0 144 261 420
Size: 144 117 159
Fragments arranged by size:
159 144 117
SacII CC_GC'GG
Cuts at: 0 24 420
Size: 24 396
SfcI C'TryA_G
Cuts at: 0 256 262 420
Size: 256 6 158
Fragments arranged by size:
256 158 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 145 420
Size: 145 275
SmaI CCC'GGG
Cuts at: 0 181 420
Size: 181 239
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 358 420
Size: 358 62
XmaI C'CCGG_G
Cuts at: 0 179 420
Size: 179 241
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI AvaI BamHI
BanII BbsI BglI BglII Bme1580I BpmI BsaI BseYI
BsiHKAI Bsp1286I BspMI BstYI Bsu36I BtgI BtgZI DrdI
EaeI Eco57MI EcoO109I FalI HincII MluI MscI MspA1I
PpiI PpuMI PspOMI PstI PvuII SacII SfcI SfiI
SmaI TaqII XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtsI
ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI