(Linear) (Six-base) MAPSORT of: KIEE5159.seq  Check: 8392  from: 1  to: 480



KIEE5159.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AccI GT'mk_AC

Cuts at:      0     258     480 
   Size:        258     222
 
ApaLI G'TGCA_C

Cuts at:      0     347     480 
   Size:        347     133
 
ApoI r'AATT_y

Cuts at:      0      26     480 
   Size:         26     454
 
BanI G'GyrC_C

Cuts at:      0     312     480 
   Size:        312     168
 
BanII G_rGCy'C

Cuts at:      0      70     105     480 
   Size:         70      35     375

  Fragments arranged by size: 

                 375      70      35
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     170     204     480 
   Size:        170      34     276

  Fragments arranged by size: 

                 276     170      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     455     462     480 
   Size:        455       7      18

  Fragments arranged by size: 

                 455      18       7
 
Bme1580I G_kGCm'C

Cuts at:      0     351     480 
   Size:        351     129
 
BsiHKAI G_wGCw'C

Cuts at:      0     351     480 
   Size:        351     129
 
Bsp1286I G_dGCh'C

Cuts at:      0      70     105     351     480 
   Size:         70      35     246     129

  Fragments arranged by size: 

                 246     129      70      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     359     480 
   Size:        359     121
 
BsrDI GCAATG_nn'

Cuts at:      0     152     164     480 
   Size:        152      12     316

  Fragments arranged by size: 

                 316     152      12
 
BstEII G'GTnAC_C

Cuts at:      0     247     480 
   Size:        247     233
 
BstYI r'GATC_y

Cuts at:      0     423     480 
   Size:        423      57
 
BstZ17I GTA'TAC

Cuts at:      0     259     480 
   Size:        259     221
 
BtgI C'CryG_G

Cuts at:      0     427     439     480 
   Size:        427      12      41

  Fragments arranged by size: 

                 427      41      12
 
ClaI AT'CG_AT

Cuts at:      0     180     480 
   Size:        180     300
 
MfeI C'AATT_G

Cuts at:      0     288     480 
   Size:        288     192
 
MslI CAynn'nnrTG

Cuts at:      0     157     480 
   Size:        157     323
 
NspI r_CATG'y

Cuts at:      0     413     480 
   Size:        413      67
 
PstI C_TGCA'G

Cuts at:      0     370     480 
   Size:        370     110
 
SfcI C'TryA_G

Cuts at:      0     366     480 
   Size:        366     114
 
SspI AAT'ATT

Cuts at:      0     357     480 
   Size:        357     123
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      46     480 
   Size:         46     434
 

 Enzymes that do cut:

     AccI    ApaLI     ApoI     BanI    BanII     BcgI    BciVI Bme1580I
  BsiHKAI Bsp1286I    BspMI    BsrDI   BstEII    BstYI  BstZ17I     BtgI
     ClaI     MfeI     MslI     NspI     PstI     SfcI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI   Bsu36I    BtgZI     BtsI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI