(Linear) (Six-base) MAPSORT of: KIEE5156.seq  Check: 2715  from: 1  to: 339



KIEE5156.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     142     339 
   Size:        142     197
 
BamHI G'GATC_C

Cuts at:      0     236     339 
   Size:        236     103
 
BanI G'GyrC_C

Cuts at:      0      51     339 
   Size:         51     288
 
BanII G_rGCy'C

Cuts at:      0     140     339 
   Size:        140     199
 
BbvCI CC'TCA_GC

Cuts at:      0     212     339 
   Size:        212     127
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       2     339 
   Size:          2     337
 
Bpu10I CC'TnA_GC

Cuts at:      0     212     339 
   Size:        212     127
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      75     156     339 
   Size:         75      81     183

  Fragments arranged by size: 

                 183      81      75
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      28      58     339 
   Size:         28      30     281

  Fragments arranged by size: 

                 281      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220     339 
   Size:        220     119
 
BseYI C'CCAG_C

Cuts at:      0      13     339 
   Size:         13     326
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     339 
   Size:        148     191
 
BsiHKAI G_wGCw'C

Cuts at:      0     303     339 
   Size:        303      36
 
Bsp1286I G_dGCh'C

Cuts at:      0     140     303     339 
   Size:        140     163      36

  Fragments arranged by size: 

                 163     140      36
 
BspHI T'CATG_A

Cuts at:      0     113     339 
   Size:        113     226
 
BsrFI r'CCGG_y

Cuts at:      0     227     339 
   Size:        227     112
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     147     339 
   Size:        147     192
 
BstYI r'GATC_y

Cuts at:      0     236     339 
   Size:        236     103
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     272     339 
   Size:        272      67
 
BtsI GCAGTG_nn'

Cuts at:      0     313     339 
   Size:        313      26
 
HaeII r_GCGC'y

Cuts at:      0     198     339 
   Size:        198     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     339 
   Size:         28      32     279

  Fragments arranged by size: 

                 279      32      28
 
MslI CAynn'nnrTG

Cuts at:      0     124     339 
   Size:        124     215
 
MspA1I CmG'CkG

Cuts at:      0     192     339 
   Size:        192     147
 
NaeI GCC'GGC

Cuts at:      0     229     339 
   Size:        229     110
 
NgoMIV G'CCGG_C

Cuts at:      0     227     339 
   Size:        227     112
 
PstI C_TGCA'G

Cuts at:      0     249     339 
   Size:        249      90
 
SfcI C'TryA_G

Cuts at:      0     245     339 
   Size:        245      94
 
StyI C'CwwG_G

Cuts at:      0      47     339 
   Size:         47     292
 

 Enzymes that do cut:

     AlfI    BamHI     BanI    BanII    BbvCI     BmrI   Bpu10I     BsaI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrFI
    BstXI    BstYI    BtgZI     BtsI    HaeII    Hin4I     MslI   MspA1I
     NaeI   NgoMIV     PstI     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI