(Linear) (Six-base) MAPSORT of: KIEE5151.seq  Check: 6631  from: 1  to: 258



KIEE5151.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AflIII A'CryG_T

Cuts at:      0      75     258 
   Size:         75     183
 
ApoI r'AATT_y

Cuts at:      0     190     258 
   Size:        190      68
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     152     258 
   Size:        152     106
 
BsaAI yAC'GTr

Cuts at:      0      76     258 
   Size:         76     182
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     258 
   Size:         37     221
 
BsmI GAATG_Cn'

Cuts at:      0      96     258 
   Size:         96     162
 
BsrDI GCAATG_nn'

Cuts at:      0     243     258 
   Size:        243      15
 
BtgI C'CryG_G

Cuts at:      0     245     258 
   Size:        245      13
 
HincII GTy'rAC

Cuts at:      0     202     258 
   Size:        202      56
 
HindIII A'AGCT_T

Cuts at:      0      46     258 
   Size:         46     212
 
MslI CAynn'nnrTG

Cuts at:      0     101     258 
   Size:        101     157
 
NcoI C'CATG_G

Cuts at:      0     245     258 
   Size:        245      13
 
PmlI CAC'GTG

Cuts at:      0      76     258 
   Size:         76     182
 
SmlI C'TyrA_G

Cuts at:      0     167     258 
   Size:        167      91
 
StyI C'CwwG_G

Cuts at:      0     224     245     258 
   Size:        224      21      13

  Fragments arranged by size: 

                 224      21      13
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      99     131     258 
   Size:         99      32     127

  Fragments arranged by size: 

                 127      99      32
 

 Enzymes that do cut:

   AflIII     ApoI    BpuEI    BsaAI    BseRI     BsmI    BsrDI     BtgI
   HincII  HindIII     MslI     NcoI     PmlI     SmlI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI