(Linear) (Six-base) MAPSORT of: KIEE5148.seq  Check: 957  from: 1  to: 192



KIEE5148.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

Acc65I G'GTAC_C

Cuts at:      0      11     192 
   Size:         11     181
 
BanI G'GyrC_C

Cuts at:      0      11     192 
   Size:         11     181
 
BanII G_rGCy'C

Cuts at:      0     119     192 
   Size:        119      73
 
BbvCI CC'TCA_GC

Cuts at:      0     130     192 
   Size:        130      62
 
BmgBI CAC'GTC

Cuts at:      0      39     192 
   Size:         39     153
 
Bpu10I CC'TnA_GC

Cuts at:      0     130     192 
   Size:        130      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      58     192 
   Size:         58     134
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     118     192 
   Size:        109       9      74

  Fragments arranged by size: 

                 109      74       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     192 
   Size:        119      73
 
BsrDI GCAATG_nn'

Cuts at:      0      81     192 
   Size:         81     111
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81     192 
   Size:         81     111
 
KpnI G_GTAC'C

Cuts at:      0      15     192 
   Size:         15     177
 
MspA1I CmG'CkG

Cuts at:      0     146     192 
   Size:        146      46
 
StyI C'CwwG_G

Cuts at:      0      29     192 
   Size:         29     163
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      36     192 
   Size:         36     156
 

 Enzymes that do cut:

   Acc65I     BanI    BanII    BbvCI    BmgBI   Bpu10I     BsaI    BseRI
 Bsp1286I    BsrDI EcoO109I     KpnI   MspA1I     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI