(Linear) (Six-base) MAPSORT of: KIEE5147.seq  Check: 2081  from: 1  to: 210



KIEE5147.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AccI GT'mk_AC

Cuts at:      0      23     210 
   Size:         23     187
 
AflIII A'CryG_T

Cuts at:      0     108     210 
   Size:        108     102
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      50      80     210 
   Size:         50      30     130

  Fragments arranged by size: 

                 130      50      30
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      87     210 
   Size:         87     123
 
BsrFI r'CCGG_y

Cuts at:      0      18     210 
   Size:         18     192
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     210 
   Size:         53     157
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     139     171     210 
   Size:        139      32      39

  Fragments arranged by size: 

                 139      39      32
 
NspI r_CATG'y

Cuts at:      0     112     210 
   Size:        112      98
 
PciI A'CATG_T

Cuts at:      0     108     210 
   Size:        108     102
 
PshAI GACnn'nnGTC

Cuts at:      0      29     210 
   Size:         29     181
 
PsiI TTA'TAA

Cuts at:      0     194     210 
   Size:        194      16
 

 Enzymes that do cut:

     AccI   AflIII     BbsI     BmrI    BsrFI     EarI    Hin4I     NspI
     PciI    PshAI     PsiI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI