Restriction map for
pFN21AE5146
(Linear) (Six-base) MAPSORT of: KIEE5146.seq Check: 2993 from: 1 to: 414
KIEE5146.orf
With 182 enzymes: SgfI *
January 29, 2014 11:48 ..
AccI GT'mk_AC
Cuts at: 0 224 414
Size: 224 190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 414
Size: 262 152
AflIII A'CryG_T
Cuts at: 0 20 287 414
Size: 20 267 127
Fragments arranged by size:
267 127 20
AgeI A'CCGG_T
Cuts at: 0 310 414
Size: 310 104
AhdI GACnn_n'nnGTC
Cuts at: 0 403 414
Size: 403 11
AlwNI CAG_nnn'CTG
Cuts at: 0 147 414
Size: 147 267
AvaI C'yCGr_G
Cuts at: 0 118 414
Size: 118 296
BanI G'GyrC_C
Cuts at: 0 153 414
Size: 153 261
BbeI G_GCGC'C
Cuts at: 0 157 414
Size: 157 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 414
Size: 250 164
BmgBI CAC'GTC
Cuts at: 0 222 414
Size: 222 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 414
Size: 109 305
Bpu10I CC'TnA_GC
Cuts at: 0 43 414
Size: 43 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 414
Size: 316 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 85 414
Size: 85 329
BsaAI yAC'GTr
Cuts at: 0 288 414
Size: 288 126
BsaHI Gr'CG_yC
Cuts at: 0 154 414
Size: 154 260
BsaWI w'CCGG_w
Cuts at: 0 249 310 414
Size: 249 61 104
Fragments arranged by size:
249 104 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 211 241 414
Size: 211 30 173
Fragments arranged by size:
211 173 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 219 414
Size: 121 98 195
Fragments arranged by size:
195 121 98
BseYI C'CCAG_C
Cuts at: 0 129 147 414
Size: 129 18 267
Fragments arranged by size:
267 129 18
BsiEI CG_ry'CG
Cuts at: 0 395 414
Size: 395 19
BsrFI r'CCGG_y
Cuts at: 0 310 414
Size: 310 104
BstAPI GCAn_nnn'nTGC
Cuts at: 0 194 414
Size: 194 220
BstEII G'GTnAC_C
Cuts at: 0 313 414
Size: 313 101
Bsu36I CC'TnA_GG
Cuts at: 0 277 414
Size: 277 137
DraIII CAC_nnn'GTG
Cuts at: 0 288 414
Size: 288 126
EaeI y'GGCC_r
Cuts at: 0 354 392 414
Size: 354 38 22
Fragments arranged by size:
354 38 22
EagI C'GGCC_G
Cuts at: 0 392 414
Size: 392 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 262 414
Size: 109 153 152
Fragments arranged by size:
153 152 109
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 414
Size: 46 368
EcoO109I rG'GnC_Cy
Cuts at: 0 171 179 414
Size: 171 8 235
Fragments arranged by size:
235 171 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 51 414
Size: 51 363
HaeII r_GCGC'y
Cuts at: 0 64 157 414
Size: 64 93 257
Fragments arranged by size:
257 93 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 57 89 389 414
Size: 57 32 300 25
Fragments arranged by size:
300 57 32 25
KasI G'GCGC_C
Cuts at: 0 153 414
Size: 153 261
MscI TGG'CCA
Cuts at: 0 356 414
Size: 356 58
MspA1I CmG'CkG
Cuts at: 0 6 129 240 380 414
Size: 6 123 111 140 34
Fragments arranged by size:
140 123 111 34 6
NarI GG'CG_CC
Cuts at: 0 154 414
Size: 154 260
NspI r_CATG'y
Cuts at: 0 24 414
Size: 24 390
PciI A'CATG_T
Cuts at: 0 20 414
Size: 20 394
PpuMI rG'GwC_Cy
Cuts at: 0 179 414
Size: 179 235
PvuII CAG'CTG
Cuts at: 0 6 240 414
Size: 6 234 174
Fragments arranged by size:
234 174 6
SanDI GG'GwC_CC
Cuts at: 0 179 414
Size: 179 235
ScaI AGT'ACT
Cuts at: 0 337 414
Size: 337 77
SfoI GGC'GCC
Cuts at: 0 155 414
Size: 155 259
SgrAI Cr'CCGG_yG
Cuts at: 0 310 414
Size: 310 104
SmaI CCC'GGG
Cuts at: 0 120 414
Size: 120 294
SmlI C'TyrA_G
Cuts at: 0 331 414
Size: 331 83
TatI w'GTAC_w
Cuts at: 0 335 414
Size: 335 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 211 243 414
Size: 211 32 171
Fragments arranged by size:
211 171 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 414
Size: 171 243
XmaI C'CCGG_G
Cuts at: 0 118 414
Size: 118 296
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AlwNI AvaI BanI
BbeI BbsI BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsrFI BstAPI
BstEII Bsu36I DraIII EaeI EagI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MscI MspA1I NarI NspI
PciI PpuMI PvuII SanDI ScaI SfoI SgrAI SmaI
SmlI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanII BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BsaBI
BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstXI
BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EarI EciI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfcI SfiI
SgfI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII Tth111I XbaI XhoI XmnI ZraI