(Linear) (Six-base) MAPSORT of: KIEE5143.seq  Check: 4142  from: 1  to: 231



KIEE5143.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     103     231 
   Size:        103     128
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      40     231 
   Size:         40     191
 
BspHI T'CATG_A

Cuts at:      0     198     231 
   Size:        198      33
 
BtsI GCAGTG_nn'

Cuts at:      0     104     231 
   Size:        104     127
 
EaeI y'GGCC_r

Cuts at:      0     189     231 
   Size:        189      42
 
HaeII r_GCGC'y

Cuts at:      0      25      43     231 
   Size:         25      18     188

  Fragments arranged by size: 

                 188      25      18
 
MscI TGG'CCA

Cuts at:      0     191     231 
   Size:        191      40
 
StyI C'CwwG_G

Cuts at:      0      58      81     231 
   Size:         58      23     150

  Fragments arranged by size: 

                 150      58      23
 
TatI w'GTAC_w

Cuts at:      0     224     231 
   Size:        224       7
 

 Enzymes that do cut:

    AlwNI    BseRI    BspHI     BtsI     EaeI    HaeII     MscI     StyI
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI