(Linear) (Six-base) MAPSORT of: KIEE5138.seq  Check: 5115  from: 1  to: 375



KIEE5138.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AfeI AGC'GCT

Cuts at:      0     137     375 
   Size:        137     238
 
AlwNI CAG_nnn'CTG

Cuts at:      0      52     375 
   Size:         52     323
 
AvaI C'yCGr_G

Cuts at:      0     248     375 
   Size:        248     127
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     229     263     375 
   Size:        229      34     112

  Fragments arranged by size: 

                 229     112      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     322     375 
   Size:        322      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      89     119     229     259     375 
   Size:         89      30     110      30     116

  Fragments arranged by size: 

                 116     110      89      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110     375 
   Size:        110     265
 
BsiHKAI G_wGCw'C

Cuts at:      0     242     375 
   Size:        242     133
 
BsiWI C'GTAC_G

Cuts at:      0      70     375 
   Size:         70     305
 
Bsp1286I G_dGCh'C

Cuts at:      0     242     375 
   Size:        242     133
 
BstYI r'GATC_y

Cuts at:      0     149     375 
   Size:        149     226
 
EaeI y'GGCC_r

Cuts at:      0      43     375 
   Size:         43     332
 
EarI CTCTTCn'nnn_

Cuts at:      0     358     375 
   Size:        358      17
 
EcoO109I rG'GnC_Cy

Cuts at:      0      81     375 
   Size:         81     294
 
HaeII r_GCGC'y

Cuts at:      0     139     375 
   Size:        139     236
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261     375 
   Size:        229      32     114

  Fragments arranged by size: 

                 229     114      32
 
MspA1I CmG'CkG

Cuts at:      0      42     220     375 
   Size:         42     178     155

  Fragments arranged by size: 

                 178     155      42
 
PvuII CAG'CTG

Cuts at:      0     220     375 
   Size:        220     155
 
SmlI C'TyrA_G

Cuts at:      0     248     375 
   Size:        248     127
 
SspI AAT'ATT

Cuts at:      0     276     375 
   Size:        276      99
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     115     225     375 
   Size:        115     110     150

  Fragments arranged by size: 

                 150     115     110
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      89     121     375 
   Size:         89      32     254

  Fragments arranged by size: 

                 254      89      32
 
XhoI C'TCGA_G

Cuts at:      0     248     375 
   Size:        248     127
 

 Enzymes that do cut:

     AfeI    AlwNI     AvaI     BcgI     BmrI    BsaXI    BseRI  BsiHKAI
    BsiWI Bsp1286I    BstYI     EaeI     EarI EcoO109I    HaeII    Hin4I
   MspA1I    PvuII     SmlI     SspI    TaqII     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI