(Linear) (Six-base) MAPSORT of: KIEE5132.seq  Check: 4633  from: 1  to: 330



KIEE5132.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     124     330 
   Size:        124     206
 
AatII G_ACGT'C

Cuts at:      0     314     330 
   Size:        314      16
 
BanI G'GyrC_C

Cuts at:      0     162     330 
   Size:        162     168
 
BanII G_rGCy'C

Cuts at:      0      45     330 
   Size:         45     285
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     146     330 
   Size:        146     184
 
BglI GCCn_nnn'nGGC

Cuts at:      0     160     330 
   Size:        160     170
 
BglII A'GATC_T

Cuts at:      0     267     330 
   Size:        267      63
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      90     330 
   Size:         90     240
 
BsaHI Gr'CG_yC

Cuts at:      0     311     330 
   Size:        311      19
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      32      62     330 
   Size:         32      30     268

  Fragments arranged by size: 

                 268      32      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7     330 
   Size:          7     323
 
Bsp1286I G_dGCh'C

Cuts at:      0      45     330 
   Size:         45     285
 
BspHI T'CATG_A

Cuts at:      0       8     330 
   Size:          8     322
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     124     330 
   Size:        124     206
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     195     330 
   Size:        195     135
 
BstYI r'GATC_y

Cuts at:      0     184     267     330 
   Size:        184      83      63

  Fragments arranged by size: 

                 184      83      63
 
EarI CTCTTCn'nnn_

Cuts at:      0      25     330 
   Size:         25     305
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      32      64     330 
   Size:         32      32     266

  Fragments arranged by size: 

                 266      32      32
 
PasI CC'CwG_GG

Cuts at:      0     145     330 
   Size:        145     185
 
ScaI AGT'ACT

Cuts at:      0     326     330 
   Size:        326       4
 
StuI AGG'CCT

Cuts at:      0     206     330 
   Size:        206     124
 
StyI C'CwwG_G

Cuts at:      0     172     330 
   Size:        172     158
 
TatI w'GTAC_w

Cuts at:      0     324     330 
   Size:        324       6
 
XbaI T'CTAG_A

Cuts at:      0     270     330 
   Size:        270      60
 
ZraI GAC'GTC

Cuts at:      0     312     330 
   Size:        312      18
 

 Enzymes that do cut:

     AarI    AatII     BanI    BanII     BbsI     BglI    BglII     BplI
    BsaHI    BsaXI    BseRI Bsp1286I    BspHI    BspMI    BstXI    BstYI
     EarI    Hin4I     PasI     ScaI     StuI     StyI     TatI     XbaI
     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XcmI
     XhoI     XmaI     XmnI