(Linear) (Six-base) MAPSORT of: KIEE5131.seq  Check: 5769  from: 1  to: 420



KIEE5131.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AlwNI CAG_nnn'CTG

Cuts at:      0      72     420 
   Size:         72     348
 
AvaI C'yCGr_G

Cuts at:      0     247     420 
   Size:        247     173
 
BglI GCCn_nnn'nGGC

Cuts at:      0      55     169     420 
   Size:         55     114     251

  Fragments arranged by size: 

                 251     114      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     420 
   Size:        346      74
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     256     280     420 
   Size:        256      24     140

  Fragments arranged by size: 

                 256     140      24
 
BseYI C'CCAG_C

Cuts at:      0     222     420 
   Size:        222     198
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      57     420 
   Size:         57     363
 
BsrFI r'CCGG_y

Cuts at:      0     126     420 
   Size:        126     294
 
BstYI r'GATC_y

Cuts at:      0      99     420 
   Size:         99     321
 
BtgI C'CryG_G

Cuts at:      0     167     420 
   Size:        167     253
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     336     371     420 
   Size:        336      35      49

  Fragments arranged by size: 

                 336      49      35
 
EaeI y'GGCC_r

Cuts at:      0     124     161     281     420 
   Size:        124      37     120     139

  Fragments arranged by size: 

                 139     124     120      37
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      73     420 
   Size:         73     347
 
EcoO109I rG'GnC_Cy

Cuts at:      0      69     252     420 
   Size:         69     183     168

  Fragments arranged by size: 

                 183     168      69
 
FspI TGC'GCA

Cuts at:      0     235     420 
   Size:        235     185
 
MspA1I CmG'CkG

Cuts at:      0     169     420 
   Size:        169     251
 
NaeI GCC'GGC

Cuts at:      0     128     420 
   Size:        128     292
 
NgoMIV G'CCGG_C

Cuts at:      0     126     420 
   Size:        126     294
 
NspI r_CATG'y

Cuts at:      0     234     318     420 
   Size:        234      84     102

  Fragments arranged by size: 

                 234     102      84
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222     420 
   Size:        222     198
 
PpuMI rG'GwC_Cy

Cuts at:      0      69     420 
   Size:         69     351
 
PspXI vC'TCGA_Gb

Cuts at:      0     247     420 
   Size:        247     173
 
SacII CC_GC'GG

Cuts at:      0     170     420 
   Size:        170     250
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     169     420 
   Size:        169     251
 
SmlI C'TyrA_G

Cuts at:      0     247     325     420 
   Size:        247      78      95

  Fragments arranged by size: 

                 247      95      78
 
SphI G_CATG'C

Cuts at:      0     234     420 
   Size:        234     186
 
StyI C'CwwG_G

Cuts at:      0     374     420 
   Size:        374      46
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     381     420 
   Size:        381      39
 
XhoI C'TCGA_G

Cuts at:      0     247     420 
   Size:        247     173
 
XmnI GAAnn'nnTTC

Cuts at:      0     403     420 
   Size:        403      17
 

 Enzymes that do cut:

    AlwNI     AvaI     BglI    BpuEI     BsaI    BseYI    BspMI    BsrFI
    BstYI     BtgI    CspCI     EaeI     EciI EcoO109I     FspI   MspA1I
     NaeI   NgoMIV     NspI    PflMI    PpuMI    PspXI    SacII     SfiI
     SmlI     SphI     StyI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII
     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XmaI     ZraI