(Linear) (Six-base) MAPSORT of: KIEE5129.seq  Check: 7222  from: 1  to: 492



KIEE5129.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 12:59  ..

AccI GT'mk_AC

Cuts at:      0      35     492 
   Size:         35     457
 
AflIII A'CryG_T

Cuts at:      0     402     492 
   Size:        402      90
 
AlwNI CAG_nnn'CTG

Cuts at:      0     283     492 
   Size:        283     209
 
ApoI r'AATT_y

Cuts at:      0     244     492 
   Size:        244     248
 
BglI GCCn_nnn'nGGC

Cuts at:      0     212     492 
   Size:        212     280
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     324     492 
   Size:        324     168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     492 
   Size:        143     349
 
BsiHKAI G_wGCw'C

Cuts at:      0     114     121     431     492 
   Size:        114       7     310      61

  Fragments arranged by size: 

                 310     114      61       7
 
Bsp1286I G_dGCh'C

Cuts at:      0     114     121     431     492 
   Size:        114       7     310      61

  Fragments arranged by size: 

                 310     114      61       7
 
BsrDI GCAATG_nn'

Cuts at:      0     470     492 
   Size:        470      22
 
BtgI C'CryG_G

Cuts at:      0     296     422     492 
   Size:        296     126      70

  Fragments arranged by size: 

                 296     126      70
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      31     492 
   Size:         31     461
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     492 
   Size:        143     349
 
HincII GTy'rAC

Cuts at:      0       6      36     492 
   Size:          6      30     456

  Fragments arranged by size: 

                 456      30       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     344     492 
   Size:        344     148
 
MspA1I CmG'CkG

Cuts at:      0      97     492 
   Size:         97     395
 
NcoI C'CATG_G

Cuts at:      0     296     422     492 
   Size:        296     126      70

  Fragments arranged by size: 

                 296     126      70
 
NspI r_CATG'y

Cuts at:      0     379     492 
   Size:        379     113
 
PsiI TTA'TAA

Cuts at:      0     143     492 
   Size:        143     349
 
SalI G'TCGA_C

Cuts at:      0      34     492 
   Size:         34     458
 
SspI AAT'ATT

Cuts at:      0     411     492 
   Size:        411      81
 
StyI C'CwwG_G

Cuts at:      0      47     296     422     492 
   Size:         47     249     126      70

  Fragments arranged by size: 

                 249     126      70      47
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     214     492 
   Size:        214     278
 
XmnI GAAnn'nnTTC

Cuts at:      0     104     492 
   Size:        104     388
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApoI     BglI     BmrI     BpmI  BsiHKAI
 Bsp1286I    BsrDI     BtgI     DrdI  Eco57MI   HincII     MmeI   MspA1I
     NcoI     NspI     PsiI     SalI     SspI     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     ZraI