(Linear) (Six-base) MAPSORT of: KIEE5128.seq  Check: 381  from: 1  to: 384



KIEE5128.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     212     384 
   Size:        212     172
 
AflIII A'CryG_T

Cuts at:      0     239     384 
   Size:        239     145
 
AlwNI CAG_nnn'CTG

Cuts at:      0     264     384 
   Size:        264     120
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     227     384 
   Size:        227     157
 
BglI GCCn_nnn'nGGC

Cuts at:      0     121     384 
   Size:        121     263
 
Bme1580I G_kGCm'C

Cuts at:      0     236     384 
   Size:        236     148
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     270     384 
   Size:        208      62     114

  Fragments arranged by size: 

                 208     114      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     288     384 
   Size:        288      96
 
BsaAI yAC'GTr

Cuts at:      0     240     384 
   Size:        240     144
 
BsaBI GATnn'nnATC

Cuts at:      0      17     384 
   Size:         17     367
 
BsaHI Gr'CG_yC

Cuts at:      0     176     384 
   Size:        176     208
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     261     291     384 
   Size:        261      30      93

  Fragments arranged by size: 

                 261      93      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     384 
   Size:        184     200
 
Bsp1286I G_dGCh'C

Cuts at:      0     236     384 
   Size:        236     148
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212     384 
   Size:        212     172
 
BstYI r'GATC_y

Cuts at:      0     344     384 
   Size:        344      40
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     282     384 
   Size:        282     102
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     270     384 
   Size:        208      62     114

  Fragments arranged by size: 

                 208     114      62
 
EcoO109I rG'GnC_Cy

Cuts at:      0     261     384 
   Size:        261     123
 
MslI CAynn'nnrTG

Cuts at:      0     163     384 
   Size:        163     221
 
MspA1I CmG'CkG

Cuts at:      0     210     333     384 
   Size:        210     123      51

  Fragments arranged by size: 

                 210     123      51
 
PasI CC'CwG_GG

Cuts at:      0     264     384 
   Size:        264     120
 
PstI C_TGCA'G

Cuts at:      0     174     384 
   Size:        174     210
 
PvuII CAG'CTG

Cuts at:      0     333     384 
   Size:        333      51
 
SfcI C'TryA_G

Cuts at:      0     170     384 
   Size:        170     214
 
StuI AGG'CCT

Cuts at:      0      28     291     384 
   Size:         28     263      93

  Fragments arranged by size: 

                 263      93      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     261     293     384 
   Size:        261      32      91

  Fragments arranged by size: 

                 261      91      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      63     261     384 
   Size:         63     198     123

  Fragments arranged by size: 

                 198     123      63
 

 Enzymes that do cut:

     AarI   AflIII    AlwNI     BbsI     BglI Bme1580I     BpmI     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI     BsgI Bsp1286I    BspMI    BstYI
    BtgZI  Eco57MI EcoO109I     MslI   MspA1I     PasI     PstI    PvuII
     SfcI     StuI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI