(Linear) (Six-base) MAPSORT of: KIEE5127.seq  Check: 5729  from: 1  to: 354



KIEE5127.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:47  ..

ApoI r'AATT_y

Cuts at:      0      24     354 
   Size:         24     330
 
BanI G'GyrC_C

Cuts at:      0     205     354 
   Size:        205     149
 
BanII G_rGCy'C

Cuts at:      0     167     354 
   Size:        167     187
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     278     354 
   Size:        278      76
 
BglI GCCn_nnn'nGGC

Cuts at:      0      51     354 
   Size:         51     303
 
BlpI GC'TnA_GC

Cuts at:      0     148     354 
   Size:        148     206
 
Bme1580I G_kGCm'C

Cuts at:      0      47     208     354 
   Size:         47     161     146

  Fragments arranged by size: 

                 161     146      47
 
BmtI G_CTAG'C

Cuts at:      0     268     354 
   Size:        268      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      76     354 
   Size:         31      45     278

  Fragments arranged by size: 

                 278      45      31
 
BsaBI GATnn'nnATC

Cuts at:      0     289     354 
   Size:        289      65
 
BsaWI w'CCGG_w

Cuts at:      0     281     354 
   Size:        281      73
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     177     354 
   Size:        177     177
 
BsiEI CG_ry'CG

Cuts at:      0     130     354 
   Size:        130     224
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     167     208     354 
   Size:         47     120      41     146

  Fragments arranged by size: 

                 146     120      47      41
 
BspHI T'CATG_A

Cuts at:      0     341     354 
   Size:        341      13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     108     354 
   Size:        108     246
 
BsrBI CCG'CTC

Cuts at:      0     152     354 
   Size:        152     202
 
BssHII G'CGCG_C

Cuts at:      0     170     354 
   Size:        170     184
 
BtgI C'CryG_G

Cuts at:      0      47     140     354 
   Size:         47      93     214

  Fragments arranged by size: 

                 214      93      47
 
EaeI y'GGCC_r

Cuts at:      0     127     137     354 
   Size:        127      10     217

  Fragments arranged by size: 

                 217     127      10
 
EagI C'GGCC_G

Cuts at:      0     127     354 
   Size:        127     227
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31      76     354 
   Size:         31      45     278

  Fragments arranged by size: 

                 278      45      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     274     354 
   Size:        274      80
 
HaeII r_GCGC'y

Cuts at:      0     148     236     354 
   Size:        148      88     118

  Fragments arranged by size: 

                 148     118      88
 
MscI TGG'CCA

Cuts at:      0     139     354 
   Size:        139     215
 
MspA1I CmG'CkG

Cuts at:      0      49      79     177     354 
   Size:         49      30      98     177

  Fragments arranged by size: 

                 177      98      49      30
 
NcoI C'CATG_G

Cuts at:      0     140     354 
   Size:        140     214
 
NheI G'CTAG_C

Cuts at:      0     264     354 
   Size:        264      90
 
PasI CC'CwG_GG

Cuts at:      0     270     354 
   Size:        270      84
 
PfoI T'CCnGG_A

Cuts at:      0       8     354 
   Size:          8     346
 
PvuII CAG'CTG

Cuts at:      0     177     354 
   Size:        177     177
 
RsrII CG'GwC_CG

Cuts at:      0     105     354 
   Size:        105     249
 
SacII CC_GC'GG

Cuts at:      0      50     354 
   Size:         50     304
 
StyI C'CwwG_G

Cuts at:      0     140     257     305     354 
   Size:        140     117      48      49

  Fragments arranged by size: 

                 140     117      49      48
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     198     354 
   Size:        198     156
 

 Enzymes that do cut:

     ApoI     BanI    BanII    BciVI     BglI     BlpI Bme1580I     BmtI
     BpmI    BsaBI    BsaWI    BseRI    BsiEI Bsp1286I    BspHI    BspMI
    BsrBI   BssHII     BtgI     EaeI     EagI  Eco57MI    EcoNI    HaeII
     MscI   MspA1I     NcoI     NheI     PasI     PfoI    PvuII    RsrII
    SacII     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII    BmgBI
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MslI     NaeI     NarI     NdeI
   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI     SacI     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI